Portal de Periódicos da CAPES

Assessment of the influence of irrelevant IgE on allergic sensitivity to two independent allergens

1979; Elsevier BV; Volume: 63; Issue: 1 Linguagem: Inglês

10.1016/0091-6749(79)90156-8

1097-6825

R. Robert Schellenberg, N ADKINSONJR,

Food Allergy and Anaphylaxis Research

Serum samples from 31 patients sensitive to both ragweed and rye grass were quantitated for IgE specific for ragweed antigen E (AgE) and rye grass group I (rye I) antigens employing the previously described radioallergosorbent test (RAST) elution technique.IgE anti-AgE ranged ,fiorn 0.4 to 320 ngiml and comprised 0.4% to 14.2% of total serum IgE.The same sera contained IgE anti-ye I ranging from 7.9 to I, 168 ngiml, comprising 1.67~ to 29.691, of total serum IgE.A new theoretical parameter (RF), representing the percent of total IgE-Fc receptors of' target cells occupied by allergen-specijc IgE, was calculated for each IgE specijicitJ by using the determinations of allergen-specific IgE antibody, total serum IgE, and assuming an equilibrium constant ,for binding of IgE molecules to basophils of 1O'W'.This "7~ occupancy" parameter correlated with skin test titration, basophil cell sensitivity, und hay fever symptom scores us well as, but not better than, the absolute values of allergen-speci$c IgE.This finding suggests thut in most atopic putients, the quantit? of irrele\~unt IgE pluys a relatively minor role in determining allergic sensitivity to a gilsen allergen The implications of this Jinding are discussed.The radioallergosorbent test (RAST) has become widely used for detection of allergen-specific IgE in human sera." 2 We recently described an RAST elution technique which quantitates allergen-specific IgE in nglml terms."This technique allows direct quantitative comparison of specific IgE antibody with nonspecific or irrelevant IgE which may compete for target cell FG receptors.Numerous investigators have examined the correlation between RAST values for serum content of allergen-specific IgE and other allergic indices.4-7Previous studies from our laboratories have shown significant correlations between IgE anti-ragweed determined by RAST and ragweed hay fever symptom