Novel Cyanine Dye-Labeled Dideoxynucleoside Triphosphates for DNA Sequencing
2002; American Chemical Society; Volume: 13; Issue: 4 Linguagem: Inglês
10.1021/bc015522y
ISSN1520-4812
AutoresR. Scott Duthie, Inta Kalve, Sui Bi Samols, Scott C. Hamilton, Inna Livshin, Mahesh Khot, Satyam Nampalli, Shiv Kumar, Carl W. Fuller,
Tópico(s)Microfluidic and Capillary Electrophoresis Applications
ResumoSingle color cyanine dye-labeled (Cy 5.0 and Cy 5.5) dideoxynucleoside-5'-triphosphates, or 'terminators', containing different spacer lengths were synthesized and evaluated for efficacy in DNA sequencing methods using a modified thermally stable DNA polymerase. The single color cyanine dye terminators were formulated into two separate sets of sequencing mixes, one for Cy 5.0 and the other for Cy 5.5, and evaluated on different automated sequencing platforms. Each set of mixes included two pyrimidine terminators with 17-atom linkers and two purine terminators with 10-atom linkers between the dye and the nucleotide. The two sets of cyanine dye-labeled terminators chosen for this cycle sequencing study produced improved band patterns with band uniformity similar to that obtained with dye-primer sequencing methods.
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