Portal de Periódicos da CAPES

Artigo Acesso aberto Produção Nacional Revisado por pares

BIBTEX RIS

Concentration by ultrafiltration and stabilization of phytase produced by solid-state fermentation

2013; Elsevier BV; Volume: 48; Issue: 2 Linguagem: Inglês

10.1016/j.procbio.2012.12.021

1873-3298

Daniel Ernesto Rodríguez-Fernández, José Luis Parada Rodríguez, Adriane Bianchi Pedroni Medeiros, Júlio César de Carvalho, Luíz Gustavo Lacerda, José A. Rodríguez-León, Carlos Ricardo Soccol,

Protein Hydrolysis and Bioactive Peptides

Ultrafiltration is an attractive downstream processing technique for concentrating enzymes and could be considered the primary step of purification. However, the efficiency of this process is often limited by protein fouling and shear-induced enzyme inactivation, which decreases permeate flux and results in the loss of enzyme activity. Although the rejection of phytase was higher than 99%, the loss of the enzyme activity was 14% during operation, indicating that the shear forces generated in the filter have significant influences on the enzyme activity. Two preparations using glycerol (25% and 35%, v/v) as a cryo-protecting agent at different temperatures were studied. The preparation containing 35% glycerol retained 70% of the initial enzyme activity at 70 °C after 1 h and had more than 3 and 6 months storage half-life at 29 °C and 4 °C, respectively.