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In vitro replication of a dam methylated and non-methylated ori-C plasmid

1984; Elsevier BV; Volume: 176; Issue: 1 Linguagem: Inglês

10.1016/0022-2836(84)90386-3

1089-8638

Patrick Hughes, Fatima-Zahra Squali-Houssaini, Patrick Forterre, Masamichi Kohiyama,

Bacterial Genetics and Biotechnology

We have examined the replication of a dam methylated and non-methylated ori-C plasmid in an in vitro ori-C dependent replication system. The results show that the non-methylated plasmid is 50% to 80% less efficient in the initiation of DNA synthesis; that the methylation state of the plasmid does not change the site of initiation at ori-C, and that in both cases initiation at this region requires the presence of exogenously furnished dnaA protein.