Portal de Periódicos da CAPES

Customized patterned substrates for highly versatile correlative light-scanning electron microscopy

2014; Nature Portfolio; Volume: 4; Issue: 1 Linguagem: Inglês

10.1038/srep07033

2045-2322

Lorena Benedetti, Elisa Sogne, Simona Rodighiero, Davide Marchesi, Paolo Milani, Maura Francolini,

Advanced Fluorescence Microscopy Techniques

Abstract Correlative light electron microscopy (CLEM) combines the advantages of light and electron microscopy, thus making it possible to follow dynamic events in living cells at nanometre resolution. Various CLEM approaches and devices have been developed, each of which has its own advantages and technical challenges. We here describe our customized patterned glass substrates, which improve the feasibility of correlative fluorescence/confocal and scanning electron microscopy.