FKBP12 Binds the Inositol 1,4,5-Trisphosphate Receptor at Leucine-Proline (1400–1401) and Anchors Calcineurin to this FK506-like Domain
1997; Elsevier BV; Volume: 272; Issue: 44 Linguagem: Inglês
10.1074/jbc.272.44.27582
ISSN1083-351X
AutoresAndrew M. Cameron, Frederick C. Nucifora, Eric T. Fung, David J. Livingston, Robert A. Aldape, Christopher A. Ross, Solomon H. Snyder,
Tópico(s)Toxin Mechanisms and Immunotoxins
ResumoThe immunophilin FKBP12 is one of the most abundant and conserved proteins in biology. It is the primary receptor for the immunosuppressant actions of the drug FK506 in whose presence FKBP12 binds to and inhibits calcineurin, disrupting interleukin formation in lymphocytes. The physiologic functions of FKBP12 are less clear, although the protein has been demonstrated to physiologically interact with the inositol 1,4,5-trisphosphate receptor (IP3R), the ryanodine receptor, and the type 1 transforming growth factor β receptor. We now report that FKBP12 binds the IP3R at residues 1400–1401, a leucyl-prolyl dipeptide epitope that structurally resembles FK506. We further demonstrate that binding to IP3R at this site enables FKBP12 to interact with calcineurin, presumably to anchor the phosphatase to IP3R and modulate the receptor's phosphorylation status. We propose that FK506 promotes an FKBP12-calcineurin interaction by mimicking structurally similar dipeptide epitopes present within proteins that use FKBP12 to anchor calcineurin to the appropriate physiologic substrates. The immunophilin FKBP12 is one of the most abundant and conserved proteins in biology. It is the primary receptor for the immunosuppressant actions of the drug FK506 in whose presence FKBP12 binds to and inhibits calcineurin, disrupting interleukin formation in lymphocytes. The physiologic functions of FKBP12 are less clear, although the protein has been demonstrated to physiologically interact with the inositol 1,4,5-trisphosphate receptor (IP3R), the ryanodine receptor, and the type 1 transforming growth factor β receptor. We now report that FKBP12 binds the IP3R at residues 1400–1401, a leucyl-prolyl dipeptide epitope that structurally resembles FK506. We further demonstrate that binding to IP3R at this site enables FKBP12 to interact with calcineurin, presumably to anchor the phosphatase to IP3R and modulate the receptor's phosphorylation status. We propose that FK506 promotes an FKBP12-calcineurin interaction by mimicking structurally similar dipeptide epitopes present within proteins that use FKBP12 to anchor calcineurin to the appropriate physiologic substrates. The immunophilins are proteins that bind the immunosuppressant drugs cyclosporin A (CsA), 1The abbreviations used are: CsA, cyclosporin A; FKBP, FK506 binding protein; CN, calcineurin; IP3R, inositol 1,4,5-trisphosphate receptor; PCR, polymerase chain reaction; aa, amino acid(s); NFAT, nuclear factor of activated T-cells; PKA, protein kinase A; AKAP, A-kinase-anchoring protein; TGF-β, transforming growth factor-β. FK506, and rapamycin with high affinity and are responsible for their therapeutic actions (for review, see Refs. 1Schreiber S.L. Science. 1991; 251: 283-287Crossref PubMed Scopus (1346) Google Scholar and 2Snyder S.H. Sabatini D.M. Nat. Med. 1995; 1: 32-37Crossref PubMed Scopus (174) Google Scholar). Cyclosporin A, a cyclic undecapeptide, binds to members of the cyclophilin family, whereas the structurally unrelated FK506 and rapamycin bind to the family of FK506 binding proteins (FKBPs). Although the cyclophilins and FKBPs lack amino acid sequence homology, both classes of proteins display peptidyl-prolyl isomerase activity, which is inhibited by their respective immunosuppressant ligands. However, inhibition of this rotamase activity does not explain immunosuppression, as some potent ligands of the immunophilins inhibit rotamase activity but lack immunosuppressant effects (3Bierer B.E. Somers P.K. Wandless T.J. Burakoff S.J. Schreiber S.L. Science. 1990; 250: 556-559Crossref PubMed Scopus (299) Google Scholar). Immunosuppression appears to stem from the binding of the drug-immunophilin complex to the calcium-activated phosphatase calcineurin (CN) to inhibit catalytic activity resulting in an accumulation of phosphorylated CN substrates (4Liu J. Farmer J.D. Lane W.S. Friedman J. Weissman I. Schreiber S.L. Cell. 1991; 66: 807-815Abstract Full Text PDF PubMed Scopus (3634) Google Scholar). One of these substrates, the transcription factor NFAT (nuclear factor of activated T-cells) in its unphosphorylated state passes from the cytoplasm to the nucleus to stimulate interleukin-2 formation. Following treatment with immunosuppressant drugs, phosphorylated levels of NFAT accumulate in the cytoplasm and are unable to enter the nucleus with the associated decrease in interleukin-2 formation being involved in immunosuppressant actions (5O'Keefe S.J. Tamura J. Kincaid R.L. Tocci M.J. O'Neill E.A. Nature. 1992; 357: 692-694Crossref PubMed Scopus (788) Google Scholar, 6Clipstone N.A. Crabtree G.R. Nature. 1992; 357: 695-697Crossref PubMed Scopus (1477) Google Scholar). Whereas pharmacologic actions of immunosuppressant drugs are readily explained by the above model, the physiologic roles of the immunophilins remain obscure despite the fact that they are among the most abundant and conserved proteins in biology. A few proteins, such as collagen and transferrin, have been shown to serve as substrates for immunophilin rotamase activity (7Steinmann B. Bruckner P. Supertifurga A. J. Biol. Chem. 1991; 266: 1299-1303Abstract Full Text PDF PubMed Google Scholar, 8Lodish H.F. Kong N. J. Biol. Chem. 1991; 266: 14835-14838Abstract Full Text PDF PubMed Google Scholar). However, it is unclear whether these are the sole or principal physiologic substrates for the rotamase activity of these proteins. Moreover, it is possible that the immunophilins bind to and regulate intracellular proteins without altering their tertiary structure by rotamase influences. Recently physiologic interactions of FKBP12 have been demonstrated with the two major intracellular calcium channels, the ryanodine receptor and the inositol 1,4,5-trisphosphate receptor (IP3R) (9Jayaraman T. Brillantes A. Timerman A.P. Fleischer S. Erdjument-Bromage H. Tempst P. Marks A.R. J. Biol. Chem. 1992; 267: 9474-9477Abstract Full Text PDF PubMed Google Scholar,10Cameron A.M. Steiner J.P. Sabatini D.M. Kaplin A.I. Walensky L.D. Snyder S.H. Proc. Natl. Acad. Sci. U. S. A. 1995; 92: 1784-1788Crossref PubMed Scopus (269) Google Scholar). In both instances FKBP12 is tightly associated with the channel and appears to be a physiologic subunit of the channel protein complex. Dissociating FKBP12 from either channel perturbs the physiologic calcium flux of the channel (10Cameron A.M. Steiner J.P. Sabatini D.M. Kaplin A.I. Walensky L.D. Snyder S.H. Proc. Natl. Acad. Sci. U. S. A. 1995; 92: 1784-1788Crossref PubMed Scopus (269) Google Scholar, 11Timerman A.P. Ogunbumni E. Freund E. Wiederrecht G. Marks A.R. Fleischer S. J. Biol. Chem. 1993; 268: 22992-22999Abstract Full Text PDF PubMed Google Scholar, 12Brillantes A.B. Ondrias K. Scott A. Kobrinsky E. Ondrisova E. Moschella M.C. Jayaraman T. Landers M. Ehrlich B.E. Marks A.R. Cell. 1994; 77: 513-523Abstract Full Text PDF PubMed Scopus (705) Google Scholar). We recently demonstrated a ternary complex between IP3R, FKBP12, and CN (13Cameron A.M. Steiner J.P. Roskams A.J. Ali S.M. Ronnett G.V. Snyder S.H. Cell. 1995; 83: 463-472Abstract Full Text PDF PubMed Scopus (451) Google Scholar). Within the complex, CN dephosphorylates IP3R especially when it has been phosphorylated by protein kinase C. The cycle of phosphorylation-dephosphorylation of IP3R in this complex appears to modulate the calcium flux of the channel. Thus, in this instance FKBP12 appears to anchor calcineurin to IP3R with regulation of IP3R function determined by phosphorylation and dephosphorylation and possibly not via a rotamase effect of FKBP12 upon IP3R. Evidence that rotamase activity of FKBP12 is not crucial to influences upon calcium channel function comes from studies by Fleischer and associates (14Timerman A.P. Wiederrecht G. Marcy A. Fleischer S. J. Biol. Chem. 1995; 270: 2451-2459Abstract Full Text Full Text PDF PubMed Scopus (129) Google Scholar) showing that FKBP12 mutants that lack rotamase activity nonetheless bind to the ryanodine receptor and influence its calcium flux. Mechanisms whereby FKBP12, IP3R, and CN interact have not been established. Because FK506 displaces IP3R from FKBP12, we speculated that IP3R functions as an “endogenous FK506,” binding to FKBP12 at the same site as FK506. Although it is a small organic molecule, FK506 serves as “molecular glue” enabling a CN-FKBP12 interaction, which does not occur in the absence of the drug ligand. Specific surface residues of FKBP12 have been identified that participate in the CN interaction once they are activated by FK506 binding (15Aldape R.A. Futer O. DeCenzo M.T. Jarrett B.P. Murcko M.A. Livingston D.J. J. Biol. Chem. 1992; 267: 16029-16032Abstract Full Text PDF PubMed Google Scholar, 16Futer O. DeCenzo M.T. Aldape R.A. Livingston D.J. J. Biol. Chem. 1995; 270: 18935-18940Abstract Full Text Full Text PDF PubMed Scopus (56) Google Scholar). According to our model an FK506 look-alike portion of IP3R would bind FKBP12 and thereby enable it to interact with CN. Alternatively, CN might associate with IP3R at a site distant from the binding of FKBP12 with interactions regulating calcium flux mediated by allosteric influences. Understanding the exact sites where IP3R binds FKBP12 and CN might clarify how FKBP12 functions in cells physiologically in the absence of drugs such as FK506. In the present study we have used the yeast two-hybrid system to identify the site in IP3R that binds FKBP12 and localized this to a specific leucyl-proline dipeptide that is also crucial for CN binding. We further demonstrate that the FKBP12·IP3R·CN complex is independent of FKBP12 rotamase activity but that unique sites on FKBP12 participate in anchoring CN to the IP3R·FKBP complex just as they participate in the formation of the CN·FK506·FKBP complex. IP3R truncation constructs 1–5 and 3b–3e were synthesized via the polymerase chain reaction (PCR) using the following primers: 1) 5′-TTGCGTCGACCATGTCTGACAAAATGTCG, 3′-CGGAATTCTCTTTATCAGAGAAAGGG; 2) 5′-GCTTGTCGACTGTGAAGGAGGATAAGG, 3′-CGGAATTCTCCAGCACCACGGCGTGC; 3) 5′-TTGCGTCGACTTTGCCCATGACTCCC, 3′-TTGAATTCCTCCTGCTGACAGTGGGC; 4) 5′-TTCGGTCGACTTTCCCAGAGAACACAGACGCC, 3′-GGACTAGTTTCAGTTCTAACACAAGG(SalI/Spe1); 5) 5′-TTGCGTCGACCAAGGATGACTTTATCTTGG, 3′-GGACTAGTCATAAATAACTCTAGCAGC(SalI/Spe1); 3b) 5′-TTGCGTCGACTTTGCCCATGACTCCC, 3′-CGGAATTCTGTGTAGACATTCTTACC; 3c) 5′-TTGCGTCGACTGAGAGGCCGAAGATACC, 3′-CGGAATTCAGACGGTCCTCCAGCGCGG; 3d) 5′-TTGCGTCGACCGTGGTGACCCACGAGGACTGC, 3′-TTGAATTCCTCCTGCTGACAGTGGGC; 3e) 5′-TTGCGTCGACAGCCTCTTTCCAGACTCTGATCC, 3′-CGGAATTCAGGCCCTGCAGATGTCTACAAGG. All constructs were inserted into the pPC97, pPC86, or pAUD6 (referred to as p3HYB in the text) yeast vector polylinker using the SalI and EcoRI restriction sites except where noted. The vectors pPC97, pPC86, and pAUD6 have been described previously (17Chevray P.M. Nathans D. Proc. Natl. Acad. Sci. U. S. A. 1992; 89: 5789-5793Crossref PubMed Scopus (481) Google Scholar, 18Marcus S. Polverino A. Barr M. Wigler M. Proc. Natl. Acad. Sci. U. S. A. 1994; 91: 7762-7766Crossref PubMed Scopus (202) Google Scholar). FKBP12 was synthesized via PCR from a rat brain library using the following primers: 5′-ACGCGTCGACCATGGGAGTACAAGTAGAAACCATCTCCCC, 3′-ATAAGAATGCGGCCGCTCATTCCAGTTTTAGAAGCTCC and inserted atSalI and NotI sites. FKBP12 mutants were obtained from Vertex Pharmaceuticals. Ryanodine receptor aa 2407–2521 was synthesized via PCR using the following primers: 5′-TTGCGTCGACTGGGGAGGAGCCCCCTGAAGAAAACC, 3′-CGGAATTCTCATGTCGGGAAGGAACCCCAC. Type 1 TGF-β receptor R4 was obtained using PCR primers: 5′-TTGCGTCGACCCACAACCGCACTGTCATTCACC, 3′-GGACTAGTCATTTTGATGCCTTCCTGTTG and inserted at the SalI and Spe1 sites. Calcineurin A, aa 1–394 was synthesized using primers: 5′-GAAGATCTTGTCCGAGCCCAAGGCGATTGATCC, 3′-ATAAGAATGCGGCCGCCTCCTTCCGGGCTGCAGCCGTGG and inserted at theBglI and NotI sites. PKA, aa 1–401 was synthesized using primers: 5′-ACGCGTCGACCTTCACGGTGGAGGTGCTGAGGCACCAGCCCG, 3′-ATAAGAATGCGGCCGCTCATGCAGTGGGCTCAACAATATCC and inserted at theSalI and NotI sites. AKAP79 was obtained by PCR using primers as follows: 5′-ACGCGTCGACAATGGAAACCACAATTTCAGAAATTCATGTAG, 3′-CGGAATTCTCACTGTAGAAGATTGTTTATTTTATTATCACTTG. The yeast two-hybrid assay for protein-protein interactions was performed as described previously (17Chevray P.M. Nathans D. Proc. Natl. Acad. Sci. U. S. A. 1992; 89: 5789-5793Crossref PubMed Scopus (481) Google Scholar). Briefly, constructs were synthesized as described above and co-transformed into both Y190 and PJ69–4A yeast strains. Yeast were plated on Leu-Trp-His+ and Leu-Trp-His− plates to assay for successful co-transformation via growth on His+ plates and protein-protein interaction via growth on His− plates. All co-transformants were also assayed by β-galactosidase nitrocellulose lift assays as described previously (17Chevray P.M. Nathans D. Proc. Natl. Acad. Sci. U. S. A. 1992; 89: 5789-5793Crossref PubMed Scopus (481) Google Scholar). Liquid β-galactosidase assays were performed as described. 2Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A., and Struhl, K. (eds) (1994)Current Protocols in Molecular Biology, pp. 13.6.1–13.6.6, John Wiley & Sons, Inc., New York. Briefly, transformed yeast were grown to saturation overnight and diluted the following morning. Diluted yeast were grown to an optical density (A 600) of 1 and pelleted. Yeast were resuspended in 1 ml of Z buffer (prepared as described) and then diluted in Z buffer at 1:10 and 1:20. Yeast were lysed with chloroform and SDS and incubated with 4 mg/mlO-nitrophenyl-β-d-galactoside at 30 °C until they turned yellow. The reaction was stopped with 1 mNa2CO3, and an A 420 was measured. β-Galactosidase activity was computed as described. Site-directed mutagenesis of IP3R was carried out using the Stratagene Chameleon double-stranded site-directed mutagenesis kit as described in the instructions. Mutating oligonucleotides were synthesized as follows: P1370S, GATGAGAACAGCTCTCTCATGTACCACATCC;P11401S, AACTCCCTGCTCTCGCTGGATGACATCG; P1401A, AACTCCCTGCTCGCGCTGGATGACATCG; P1401Q, AACTCCCTGCTCCAGCTGGATGACATCG; P1401K, AACTCCCTGCTCAAGCTGGATGACATCG; P1416S, GAGGACTGCATCTCTGAGGTTAAAATTGC; P1416A, GAGGACTGCATCGCTGAGGTTAAAATTGC, and to knock out the AATII site as described: TAAGTAAGTAAGCCGTCGAGCTCTAAGTAAGTAACG. A third yeast expression vector, pAUD6 (referred to as p3HYB in Fig. 5 and has been described previously (18Marcus S. Polverino A. Barr M. Wigler M. Proc. Natl. Acad. Sci. U. S. A. 1994; 91: 7762-7766Crossref PubMed Scopus (202) Google Scholar)), was obtained and used for three-hybrid assay studies. The pAUD6 vector contains the URA3 gene (orotidine-5′-phosphate decarboxylase) allowing for selection of triply transformed yeast colonies on Leu-Trp-Ura− plates. PJ69–4A strain but not Y190 are Ura− and were thus used for three-hybrid assays. Three constructs were expressed instead of two as above, and co-transformants were assayed for their ability to grow on Leu-Trp-Ura-His− plates via the liquid β-galactosidase assay as described above. FK506 was included in the agar plates at 1 μm where indicated. IP3R is one of the largest membrane proteins in biology comprising 2,749 amino acids. The receptor is suggested to encompass six transmembrane domains in the carboxyl-terminal portion of the molecule that participates in the formation of the calcium ion pore, whereas a very large N-terminal portion of the molecule is free in the cytoplasm (for review, see Ref. 19Furuichi T. Mikoshiba K. J. Neurochem. 1995; 64: 953-960Crossref PubMed Scopus (181) Google Scholar). We systematically truncated IP3R into overlapping successive regions and examined its interactions with FKBP12 in the yeast two-hybrid system. An initial series of truncations reveals the binding domain to be in the central modulatory portion of IP3R, amino acids 942–1770 (Fig.1, A and B). Further truncations of this region localize the binding site to 112 amino acids, 1349–1460 (Fig. 1, C and D). It is possible to speculate where FKBP12 binds within this 112 amino acid portion of the IP3R based on extensive x-ray crystallographic modeling and in vitro studies of FKBP12-substrate interactions. FK506 has been proposed to mimic protein ligands to, or substrates of FKBP12 with the pyranose rings, α-ketoamide functions, and homoprolyl moieties of the natural product showing structural similarities to the transition state structures forcis-trans isomerization of leucyl-prolyl and valyl-prolyl substrates, which are optimal rotamase substrates for FKBP12 (20Ikeda Y. Schultz W. Clardy J. Schreiber S.L. J. Am. Chem. Soc. 1994; 116: 4143-4144Crossref Scopus (24) Google Scholar, 21Albers M.W. Walsh C.T. Schreiber S.L. J. Org. Chem. 1990; 55: 4984-4986Crossref Scopus (128) Google Scholar). The 1349–1460 region of IP3R contains three proline residues, only one of which is preceded by either a leucine or a valine residue. Proline 1401 is preceded by a leucine. Mutations of the other two prolines contained within this region, which occur at the 1370 and 1416 positions and are preceded by a serine and an isoleucine, respectively, have no effect upon FKBP12 binding activity, whereas four distinct mutations of proline 1401 eliminate the IP3R-FKBP12 interaction (Fig.2). We wondered whether the IP3R sequence responsible for FKBP12 binding is conserved in the other proteins known to bind FKBP12. Besides IP3R and the ryanodine receptor, the type 1 TGF-β receptor has been shown to interact with FKBP12 (22Wang T. Donahoe P.K. Zervos A.S. Science. 1994; 265: 674-676Crossref PubMed Scopus (312) Google Scholar). Three subtypes of IP3R, the ryanodine receptor, and type-1 TGF-β receptors possess the proline corresponding to Pro-1401 of IP3R (Fig.3). Type 2 TGF-β receptors lack the SGSGSGLP motif present in type 1 receptors (23Derynck R. Trends Biochem. Sci. 1994; 19: 533-548Abstract Full Text PDF PubMed Scopus (277) Google Scholar) and also fail to interact with FKBP12 (22Wang T. Donahoe P.K. Zervos A.S. Science. 1994; 265: 674-676Crossref PubMed Scopus (312) Google Scholar, 24Wang T. Li B.-Y. Danielson P.D. Shah P.C. Rockwell S. Lechleider R.J. Martin J. Manganaro T. Donahoe P.K. Cell. 1996; 86: 435-444Abstract Full Text Full Text PDF PubMed Scopus (310) Google Scholar). In the ryanodine receptor this proline is preceded by a valine, whereas in the other receptors it is preceded by a leucine. In all of the receptors that bind FKBP12, the proline is followed by a leucine. Because FKBP12 seems to associate with and regulate calcium flux of the IP3R and the ryanodine receptor in a similar fashion, one might expect FKBP12 to bind to the two channels at an analogous site. The ryanodine receptor comprises 5,037 amino acids and is the largest ion channel currently known. We constructed a 114-amino acid fragment of the ryanodine receptor (aa 2, 407–2, 520) comprising the domain that corresponds to the portion of IP3R that binds to FKBP12. This small fragment binds robustly to FKBP12 in a yeast two-hybrid assay (Fig. 3). The finding that FKBP12 binds to IP3R at a discrete leucyl-proline dipeptide is consistent with the observedin vitro rotamase substrate specificity of FKBP12 (21Albers M.W. Walsh C.T. Schreiber S.L. J. Org. Chem. 1990; 55: 4984-4986Crossref Scopus (128) Google Scholar). This would suggest that FKBP12 regulates IP3R channel function by altering the conformation of the protein via rotamase influences. However, Fleischer and colleagues (14Timerman A.P. Wiederrecht G. Marcy A. Fleischer S. J. Biol. Chem. 1995; 270: 2451-2459Abstract Full Text Full Text PDF PubMed Scopus (129) Google Scholar) found that FKBP12 mutants devoid of rotamase activity retain the ability to associate with and modulate the Ca2+ flux properties of the ryanodine receptor. We examined whether the observed association of FKBP12 and IP3R requires FKBP12 rotamase activity. We compared wild-type FKBP12 with four previously described FKBP12 mutants, one that is devoid of rotamase activity and another that displays half that of wild-type FKBP12. 3M. T. DeCenzo, S. T. Park, B. P. Jarrett, R. A. Aldape, O. Futer, M. A. Murcko, and D. J. Livingston, submitted for publication. Each FKBP12 mutant interacts robustly with the IP3R regardless of its peptidyl-prolyl rotamase activity (Fig.4). Expression of these FKBP12 mutants and subsequent analysis of their ability to interact with and modulate IP3R that has been purified and “stripped” of endogenously associated wild-type FKBP12 is currently underway. In our earlier study (13Cameron A.M. Steiner J.P. Roskams A.J. Ali S.M. Ronnett G.V. Snyder S.H. Cell. 1995; 83: 463-472Abstract Full Text PDF PubMed Scopus (451) Google Scholar) establishing a ternary complex of FKBP12, IP3R, and CN, the sites of interaction of the three proteins were unclear. It was not established in that study whether CN was associating with the IP3R·FKBP12 complex via an FKBP12 anchor or at an allosteric site on IP3R. We set out to distinguish between these two possibilities using the yeast two-hybrid system and a modification of it. We first generated a CN-A construct consisting of amino acids 1–394 that contains the FKBP12 binding portion of the molecule (26Griffith J.P. Kim J.L. Kim E.E. Sintchak M.D. Thomson J.A. Fitzgibbon M.J. Fleming M.A Caron P.R. Hsiao K. Navia M.A. Cell. 1995; 82: 507-522Abstract Full Text PDF PubMed Scopus (774) Google Scholar, 27Kissinger C.R. Parge H.E. Knighton D.R. Lewis C.T. Pelletier L.A. Tempczyk A. Kalish V.J. Tucker K.D. Showalter R.E. Moomaw E.W. Gastinel L.N. Habuka N. Chen X. Maldonado F. Barker J.E. Bacquet R. Villafranca E. Nature. 1995; 378: 641-644Crossref PubMed Scopus (699) Google Scholar). We tested the ability of CN to interact directly with IP3R using this construct and observe no direct CN-IP3R interaction with any of our IP3R constructs including IP3R aa 1349–1460 (data not shown). CN likewise fails to interact with FKBP12 in the yeast two-hybrid system until FK506 is added to the agar plates during preparation (Fig.5) as previously observed (28Clipstone N.A. Fiorentino D.F. Crabtree G.R. J. Biol. Chem. 1994; 269: 26431-26437Abstract Full Text PDF PubMed Google Scholar). We obtained a third vector suitable for expressing proteins in one of the yeast strains that we were using in our two-hybrid assay (see “Experimental Procedures”). With this vector, however, expressed proteins are not synthesized as part of a GAL4 transcription factor fusion protein. We attempted to recreate a three protein interaction in the yeast system using a known ternary complex: the CN·PKA·AKAP (A-kinase-anchoring protein) complex described by Scott and associates (29Coghlan V.M. Perrino B.A. Howard M. Langeberg L.K. Kicks J.B. Gallatin W.M. Scott J.D. Science. 1995; 267: 108-111Crossref PubMed Scopus (529) Google Scholar). CN has no direct affinity for PKA in a traditional yeast two-hybrid system assay (data not shown), but the two molecules are brought together in the presence of the anchoring protein AKAP79 (Fig.5). We next investigated whether the IP3R, aa 1349–1460, mimicks FK506 by promoting an FKBP12-CN interaction in this yeast three-hybrid system. Replacing FK506 with the 112 amino acid domain of IP3R that binds FKBP12 results in a robust FKBP12-CN interaction (Fig. 5). Mutants of FKBP12 have been developed that vary in their ability to participate in binding CN (15Aldape R.A. Futer O. DeCenzo M.T. Jarrett B.P. Murcko M.A. Livingston D.J. J. Biol. Chem. 1992; 267: 16029-16032Abstract Full Text PDF PubMed Google Scholar, 16Futer O. DeCenzo M.T. Aldape R.A. Livingston D.J. J. Biol. Chem. 1995; 270: 18935-18940Abstract Full Text Full Text PDF PubMed Scopus (56) Google Scholar).3 Some mutants with almost no rotamase activity still bind CN tightly in the presence of FK506, i.e. FKBP(W59A), whereas others retain rotamase activity but are unable to bind CN when FK506 is present,i.e. FKBP(R42K/H87V) (Fig. 5). We examined the ability of certain of these mutants to support the ternary complex of FKBP12, CN, and IP3R. A mutant previously demonstrated to be almost devoid of rotamase activity, but binds CN, interacts robustly in our yeast three-hybrid assay (Fig. 5). This further supports the finding that rotamase activity is not crucial for the ternary complex. On the other hand, a mutant that is known not to interact with CN, although it possesses rotamase activity, fails to bind in the yeast three-hybrid system (Fig. 5). In the present study we have localized the exact peptide sequence within IP3R that binds to FKBP12 as a leucyl-proline dipeptide. An analogous leucyl-prolyl or valyl-prolyl sequence is conserved throughout all subtypes of IP3R, the ryanodine receptor, and the type 1 TGF-β receptors shown to interact with FKBP12 indicating that this sequence may represent a universal ligand selective for FKBP12 binding. The leucyl-proline and valyl-prolyl sequences correspond well with the substrate specificity that Schreiber and associates (21Albers M.W. Walsh C.T. Schreiber S.L. J. Org. Chem. 1990; 55: 4984-4986Crossref Scopus (128) Google Scholar) have shown to be optimal for in vitroFKBP12-peptide interactions. There are several limitations to our current study utilizing the yeast two- and three-hybrid interaction trap assays. First, although it is now routine to use this assay to map interacting domains within proteins known to associate, this method does not provide for quantification of the affinity of the interaction being studied. Some insight into this question has been attained in previous studies in which relatively high concentrations of the drug FK506 were required to disrupt the FKBP-IP3R interaction (EC50 10–100 nm), and in the case of the FKBP-ryanodine receptor interaction, Fleischer and colleagues (14Timerman A.P. Wiederrecht G. Marcy A. Fleischer S. J. Biol. Chem. 1995; 270: 2451-2459Abstract Full Text Full Text PDF PubMed Scopus (129) Google Scholar) have predicted an EC50 of 0.30 μm (13Cameron A.M. Steiner J.P. Roskams A.J. Ali S.M. Ronnett G.V. Snyder S.H. Cell. 1995; 83: 463-472Abstract Full Text PDF PubMed Scopus (451) Google Scholar). Likewise, although the FKBP-IP3R protein-protein interaction has been demonstrated in previous reports by traditional protein biochemistry methodology including co-purification, co-immunoprecipitation, and direct binding assays (10Cameron A.M. Steiner J.P. Sabatini D.M. Kaplin A.I. Walensky L.D. Snyder S.H. Proc. Natl. Acad. Sci. U. S. A. 1995; 92: 1784-1788Crossref PubMed Scopus (269) Google Scholar, 13Cameron A.M. Steiner J.P. Roskams A.J. Ali S.M. Ronnett G.V. Snyder S.H. Cell. 1995; 83: 463-472Abstract Full Text PDF PubMed Scopus (451) Google Scholar), the current study does not repeat those techniques when identifying the site of interaction. Rather the yeast two-hybrid assay is employed to identify the site of interaction, and noninteracting portions of these proteins and mutant constructs are used to ensure the specificity of the interaction. Ideally, the entire 310-kDa IP3R would be expressed as a recombinant protein with and without mutations at proline 1401 and tested for its ability to interact with FKBP in a biochemical assay. These ambitious experiments are currently underway to confirm the data obtained in the present round of yeast two-hybrid experiments. Finally, our data reported here do not speak to how small a domain of IP3R is required to mediate FKBP binding. We have shown that a 112-amino acid truncation of IP3R including proline 1401 is sufficient for FKBP binding and that proline 1401 is necessary. In an attempt to further narrow down how many residues surrounding Pro-1401 are required for FKBP interaction, we have generated an 11-amino acid peptide consisting of IP3R aa 1396–1406. This peptide was not able to support the FKBP-CN interaction in a binding assay, implying that three-dimensional structural motifs contained with the surrounding 112 amino acids also participate in the IP3R·FKBP·CN complex (data not shown). The crucial leucyl-proline sequence in IP3R implies that FKBP12 regulates IP3R by its rotamase activity. However, we showed that rotamase activity is not required for FKBP12 binding to IP3R, and Fleischer and colleagues (14Timerman A.P. Wiederrecht G. Marcy A. Fleischer S. J. Biol. Chem. 1995; 270: 2451-2459Abstract Full Text Full Text PDF PubMed Scopus (129) Google Scholar) have likewise shown that this rotamase activity is not necessary for the association of FKBP12 with or modulation of the ryanodine receptor. More important than rotamase activity in regulating IP3R function is the ability of FKBP12 to serve as a scaffold linking CN to IP3R. Because of the numerous similarities of the ryanodine receptor and IP3R, we suggest that FKBP12 also regulates the ryanodine receptor function by a link to CN. FKBP12 has been proposed to play such an anchoring role when it associates with the type 1 TGF-β receptor (24Wang T. Li B.-Y. Danielson P.D. Shah P.C. Rockwell S. Lechleider R.J. Martin J. Manganaro T. Donahoe P.K. Cell. 1996; 86: 435-444Abstract Full Text Full Text PDF PubMed Scopus (310) Google Scholar). Previous findings from our laboratory demonstrated that calcineurin complexed to IP3R via FKBP12 was catalytically active (13Cameron A.M. Steiner J.P. Roskams A.J. Ali S.M. Ronnett G.V. Snyder S.H. Cell. 1995; 83: 463-472Abstract Full Text PDF PubMed Scopus (451) Google Scholar). Interestingly, recent x-ray crystallographic evidence suggests that FK506-FKBP12 binds to calcineurin at a site removed from the phosphatase active site of the enzyme and therefore would not be likely to inhibit phosphatase activity (26Griffith J.P. Kim J.L. Kim E.E. Sintchak M.D. Thomson J.A. Fitzgibbon M.J. Fleming M.A Caron P.R. Hsiao K. Navia M.A. Cell. 1995; 82: 507-522Abstract Full Text PDF PubMed Scopus (774) Google Scholar). Rather, binding of FK506-FKBP12 seems to sterically block the association with and subsequent dephosphorylation of some substrates (i.e. NFAT) while not affecting or actually promoting the dephosphorylation by calcineurin of others (4Liu J. Farmer J.D. Lane W.S. Friedman J. Weissman I. Schreiber S.L. Cell. 1991; 66: 807-815Abstract Full Text PDF PubMed Scopus (3634) Google Scholar). If calcineurin is shown to be the cytoplasmic protein associated with the TGF-β receptor·FKBP12 complex as proposed then the recent data of Wang et al. (22Wang T. Donahoe P.K. Zervos A.S. Science. 1994; 265: 674-676Crossref PubMed Scopus (312) Google Scholar, 24Wang T. Li B.-Y. Danielson P.D. Shah P.C. Rockwell S. Lechleider R.J. Martin J. Manganaro T. Donahoe P.K. Cell. 1996; 86: 435-444Abstract Full Text Full Text PDF PubMed Scopus (310) Google Scholar) indicate calcineurin would be catalytically active in such a complex as well. Although FKBP12 has been studied extensively, its physiologic substrates have not been well characterized. No major normal function of a protein has been shown to be regulated by the rotamase activity of FKBP12. Thus, it is possible that the anchoring function of FKBP12 represents its major physiologic role. Anchoring of CN and perhaps other phosphatases and kinases to appropriate substrates might regulate phosphorylation-dephosphorylation events. Such a model is reminiscent of the AKAP, which anchors CN to appropriate substrates at the postsynaptic density via an FKBP-like domain within its sequence. Besides anchoring PKA and CN to their appropriate subcellular locales, AKAP serves as a scaffolding anchor for protein kinase C (25Klauck T.M. Faux M.C. Labudda K. Langeberg L.K. Jaken S. Scott J.D. Science. 1996; 271: 1589-1592Crossref PubMed Scopus (483) Google Scholar). The AKAP complex thus includes both phosphatase and kinase enzymes. By analogy, there might exist a physiologic quaternary complex including a protein kinase with FKBP12, IP3R and CN. In support of this model, our earlier study showed that calcium flux of IP3R was most strikingly regulated by interactions between calcineurin and protein kinase C (13Cameron A.M. Steiner J.P. Roskams A.J. Ali S.M. Ronnett G.V. Snyder S.H. Cell. 1995; 83: 463-472Abstract Full Text PDF PubMed Scopus (451) Google Scholar). Conceivably protein kinase C exists in a quaternary complex with IP3R, FKBP12, and CN. Within the ternary complex, IP3R seems to mimic the role of FK506 in promoting the association of calcineurin with FKBP12. Indeed, it is remarkable that a small organic molecule such as FK506 could contain two distinct domains to participate in binding to two different proteins, something that one would expect a protein such as IP3R to accomplish more efficiently. Our experiments with various truncations of IP3R establish that the domain of IP3R that is responsible for FKBP12 binding also enables bound FKBP12 to associate with calcineurin. The high affinity and selectivity of FK506 binding to FKBP12 has suggested to many investigators the existence of an endogenous FK506-like ligand, conceivably a small peptide, analogous to the enkephalins serving as endogenous ligands for the opiate receptor. Since the chemical structure of FK506 resembles a leucyl-proline dipeptide, it would not be surprising if the endogenous FK506-like ligand would comprise such a structure. Indeed, our results indicate that the leucyl-proline dipeptide within IP3R represents that endogenous ligand, except it is buried within a large protein. Situating the endogenous ligand within a large protein enables it to carry out a bridging function linking calcineurin via FKBP12 to an appropriate cellular substrate of its phosphatase activity (Fig.6). We thank Phil Heiter for the pAUD6 yeast vector, Philip James for use of the PJ69–4A ura3–52-yeast strain, and Antonis S. Zervos for R4 type 1 TGF-β receptor cDNA.
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