Quantitative determination of quinic acid and derivatives by high-performance liquid chromatography after derivatization with p-bromophenacyl bromide
1980; Elsevier BV; Volume: 190; Issue: 2 Linguagem: Inglês
10.1016/s0021-9673(00)88246-1
ISSN1873-3778
AutoresL.J. Nagels, C. Debeuf, Eddy L. Esmans,
Tópico(s)Advanced Chemical Sensor Technologies
ResumoQuantitative determinations of quinic, shikimic and glycolic acide have been performed by reversed-phase high-performance liquid chormatography using p-bromophenacyl bromide as a visualizing reagent. The reactions are carried out in N,N-dimethylformamide (DMF) with KF as a catalyst. Linear calibration curves are obtained in the concentration range 0.1–2 mg acide per ml DMF. Spectroscopic and chromatographic properties of the pbromophenacyl bromide derivatives of quinic, shikimic, dehydroshikimic and glycolic acid are given. A quantitative analysis of quinic acid in plant material is demonstrated.
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