Portal de Periódicos da CAPES

Human brain glucose metabolism mapping using multislice 2D1H-13C correlation HSQC spectroscopy

2000; Wiley; Volume: 43; Issue: 4 Linguagem: Inglês

10.1002/(sici)1522-2594(200004)43

1522-2594

Hidehiro Watanabe, M. Umeda, Yasutoshi Ishihara, Kazuya Okamoto, Koichi Oshio, Tomoyuki Kanamatsu, Yuiko Tsukada,

Spectroscopy Techniques in Biomedical and Chemical Research

A method for multivolume 2D 1H-13C correlation spectroscopy, multislice heteronuclear single quantum coherence (HSQC), is proposed. This permits human brain metabolism from glucose to amino acids to be followed using a 2-T whole-body scanner. The modifications from the conventional HSQC are that the 180°(13C) and 180°(1H) pulses are separated in time in the preparation period and that the 180°(13C) pulse is applied at 1/(4JCH) before the 90°(1H) polarization transfer (PT) pulse. The preparation (echo) time can be set longer than 1/(2JCH) so that, even in a whole-body system, slice-selective pulses and gradients can be applied. Another modification is that the 90°(1H) reverse PT pulses after the creation of 2IzSz are used as multislice pulses. The time-course of glutamate C4 could be followed with 15-min temporal resolution from the HSQC spectra obtained from the brains of volunteers after the oral administration of glucose C1, and the maximum S/N was 3. Magn Reson Med 43:525–533, 2000. © 2000 Wiley-Liss, Inc.