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Synthesis of Cholesteryl Supports and Phosphoramidite for Automated DNA Synthesis of Triple-Helix Forming Oligonucleotides (Tfos)

1993; Taylor & Francis; Volume: 12; Issue: 8 Linguagem: Inglês

10.1080/07328319308018556

2332-3892

Huynh Vu, Pradeep Singh, Laurie Lewis, Joseph G. Zendegui, Krishna Jayaraman,

RNA Interference and Gene Delivery

Abstract DMT-Cholesteryl succinylamino solid supports (CPG, loading: 33 μmole/gram and TentaGel loading: 152 μmole/gram) and DMT-cholesteryl phosphoramidite were prepared for use in automated DNA synthesis of cholesteryl modified TFOs in the synthesis scales from 0.2 to 300 μmole. The modified TFOs were found to have a 5 to 50 fold increase in their uptake properties.