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4-Methylumbelliferyl-glycosides as fluorescence probes of sugar-binding sites on lectin molecules: Spectral properties and dependence of fluorescence on polarity and viscosity

1984; Elsevier BV; Volume: 232; Issue: 2 Linguagem: Inglês

10.1016/0003-9861(84)90584-8

1096-0384

Monique Decastel, Michel Vincent, Khushi L. Matta, Jean‐Pierre Frénoy,

Molecular Sensors and Ion Detection

The spectral properties of 4-methylumbelliferyl-glycosides (MeUmb-glycosides) were investigated in order to assess their usefulness as probes of the microenvironment of sugar binding sites on lectin molecules. It was shown that the abnormally high values for fluorescence polarization of free MeUmb-glycosides (from 0.07 to 0.251) were due neither to their molecular size nor to the blockade of their movement, but to the short lifetimes (<0.55 ns) of the excited state of these compounds. Working essentially with two MeUmb-monosaccharides and one MeUmb-disaccharide (MeUmb-α-d-galactopyranoside, MeUmb-β-d-galactopyranoside, and MeUmb-2-acetamido-2-deoxy-3-O-(β-d-galactopyranosyl)-β-d-galactopyranoside) which were solubilized in various solvents, it was demonstrated that solvent polarity and viscosity definitely affected the fluorescence intensity of MeUmb-glycosides. A low-polarity medium reduced this intensity, and high viscosity enhanced it. The implications of these findings are discussed in relation to the variations in the fluorescence intensity of MeUmb-glycosides when these compounds were bound to lectins.