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Possible binding site of thyrotropin binding inhibitor immunoglobulin (TBII) on the thyrotropin (TSH) receptor, which is different from TSH binding site

1990; Elsevier BV; Volume: 172; Issue: 2 Linguagem: Inglês

10.1016/0006-291x(90)90705-r

1090-2104

T Piraphatdist, Hideo Sugawa, Daisuke Inoue, Tetsuya Enomoto, Toru Mori, Hiroo Imura,

Thyroid Disorders and Treatments

A synthetic decapeptide, P-194, which has the sequence No. 103 to 111 of hTSH receptor structure with an additional N-terminal tyrosine, did not bind TSH nor affected its receptor binding and thyroid stimulating activity. Preincubation of P-194 with sera from thyroid patients caused a significant decrease in TBII activity in almost all 12 TBII positive sera and an increase of thyroid stimulating activity in 3 of 7 Graves' IgG studied. In addition, [125I] P-194 bound to serum IgG fraction from thyroid patients with a positive correlation with TBII (N=35, r=0.509, p<0.01). The P-194 portion may be, at least a part of, TBII binding site distinct from the TSH binding site on the TSH receptor.