An effective method for silver-staining DNA in large numbers of polyacrylamide gels
2008; Elsevier BV; Volume: 385; Issue: 1 Linguagem: Inglês
10.1016/j.ab.2008.10.024
ISSN1096-0309
AutoresS. O. Byun, Qian Fang, Huitong Zhou, Jon G. H. Hickford,
Tópico(s)RNA modifications and cancer
ResumoSilver-staining of nucleic acid has been used for various biological analyses, including polymerase chain reaction–single-strand conformation polymorphism (PCR–SSCP) analysis. A variety of methods have been described, but these methods are not that effective for staining more than a few PCR–SSCP gels, especially rapidly and with high sensitivity, because they include a number of time-consuming or hazardous manual steps that are often time dependent. Here we report a silver-staining method that can efficiently stain up to 14 gels at one time and with a detection limit of approximately 10 pg of DNA/mm2, which is comparable to other methods.
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