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Titration of tannin via alkaline phosphatase activity

1991; Elsevier BV; Volume: 192; Issue: 2 Linguagem: Inglês

10.1016/0003-2697(91)90536-3

1096-0309

Yitzhak Ittah,

Phytase and its Applications

An enzyme assay for tannin is described. It is based on the following steps: (i) bovine serum albumin (BSA) is absorbed onto polystyrene microplates; (ii) tannin is bound to the BSA-coated plates; (iii) alkaline phosphatase is then interacted with the free tannin-binding sites. The method takes advantage of the multiple hydroxyl groups of tannin which can associate more than one ligand, e.g., proteins. A pH-dependent dynamic equilibrium sets up between bound and unbound tannin and is controlled only by its initial concentration.