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Antidiabetic activity and phytochemical screening of crude extract of Stevia rebaudiana in alloxan-induced diabetic rats

2010; Medknow; Volume: 2; Issue: 4 Linguagem: Inglês

10.4103/0974-8490.69128

0976-4836

BK Roy, RS Kujur, Vishakha Singh, Mahendra Ram, HarlokeshNarayan Yadava, KK Singh, Suruchi Kumari,

Regulation of Appetite and Obesity

Pharmacognosy Research,2010,2,4,258-263.DOI:10.4103/0974-8490.69128Published:September 2010Type:Original ArticleAuthors:R. S. Kujur Vishakha Singh, Mahendra Ram, Harlokesh Narayan Yadava, K K Singh, Suruchi Kumari, and B. K. Roy Author(s) affiliations:RS Kujur1, Vishakha Singh1, Mahendra Ram1, Harlokesh Narayan Yadava2, KK Singh3, Suruchi Kumari1, BK Roy1 1Department of Pharmacology and Toxicology, College of Veterinary Science and A.H., Birsa Agricultural University, Ranchi - 834 006, India 2Department of Pharmacology, I.S.F. College of Pharmacy, Moga - 142 001, Punjab, India 3Department of Veterinary Pathology, College of Veterinary Science and A.H., Birsa Agricultural University, Ranchi - 834 006, India Abstract:Background: Stevia rebaudiana regulates blood sugar, prevents hypertension and tooth decay. Other studies have shown that it has antibacterial as well as antiviral property. Methods: Preliminary phytochemical screening of aqueous, ether and methanolic extracts of S. rebaudiana was done. Acute and sub-acute toxicity were conducted on twenty four Albino rats, divided into one control (Group I) and three treatment groups viz. aqueous extract (Group II), ether extract (Group III) and methanolic extract (Group IV). For the study of antidiabetic effect of S. rebaudiana rats were divided into seven groups (n=6). Diabetes was induced by a single dose of 5% alloxan monohydrate (125 mg/kg, i.p.) after 24 hour fasting.Blood samples were analysed on day 0, 1, 5, 7, 14 and 28. Results: Phytochemical tests showed presence of different kinds of phyto-constituents in aqueous, ether and methanol extract of Stevia rebaudiana leaves. Daily single dose (2.0 g/kg) administration of aqueous extract (A.E.) , ether extract (E.E.) and methanol extract (M.E.) for 28 days of S. rebaudiana could not show any significant change in ALT and AST levels in rats. Blood sugar level was found to be decreased on day 28 in groups of rats treated with A.E., E.E. and M.E. of S. rebaudiana. Conclusion: The extracts of Stevioside rebaudiana could decrease the blood glucose level in diabetic rats in time dependent manner. Keywords:Aqueous extract, Diabetes, Ether extract, Methanol extract, Rats, Stevia rebaudiana, Sub-acute toxicityView:PDF (688.73 KB)