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In vitro uptake of C14-labeled alanine and glucose by Ascaridia galli (nematoda) of chickens

1963; Elsevier BV; Volume: 14; Issue: 1 Linguagem: Inglês

10.1016/0014-4894(63)90007-9

1090-2449

Norman F. Weatherly, M. F. Hansen, H. C. Moser,

Nematode management and characterization studies

This is a study of the permeability of the cuticle of Ascaridia galli to dl-alanine-1-C14 and d-glucose-1-C14. Celloidin was used to occlude the natural body openings of male and female worms, as thread-ligatures were considered to produce excessive tissue damage. Celloidin-coated and noncoated worms were exposed to either 15 ml (9.3 × 10−5M) of labeled alanine (5.0 μc) or 15 ml (1.1 × 10−4M) of labeled glucose (5.0 μc) for 1 or 2 hours. The cuticle proved permeable to both compounds. A portion of the total uptake resulted from cuticular absorption. It was hypothesized that the remainder resulted from ingestion. The amount of alanine and glucose absorbed by the coated worms was a function of the exposed body surface. Approximately 2.6 times more glucose than alanine was absorbed. Fasting had little effect on cuticular absorption by coated worms. However, the amount of uptake by noncoated worms was conditioned by fasting. All worms exposed to the labeled compounds were analyzed for C14 distribution. Analyses of noncoated worms exposed to alanine showed that they incorporated 23 and 32% of the C14 into the lipid and water fractions, respectively. The corresponding percentages for the coated worms were 39 and 22. This inverse relationship may have resulted from the slow absorption of ingested alanine. Noncoated worms exposed to glucose contained 19 and 38% of the C14 in the lipid and water fractions, respectively. The corresponding percentages for the coated worms were 22 and 35. Rapid phosphorylation and subsequent absorption of the glucose seemingly prevented the inverse relationship reported for alanine. Isolated tissue analyses of coated worms indicated that the compounds passed through the cuticle, because approximately 50% of the recovered C14 was in the perienteric fluid.