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Gas chromatography/mass spectrometry as a suitable alternative technique to evaluate the ability ofStreptomyces to degrade lignin from lignocellulosic residues

1998; Wiley; Volume: 12; Issue: 22 Linguagem: Inglês

10.1002/(sici)1097-0231(19981130)12

1097-0231

M. J. Hernández‐Coronado, Manuel Hernández, Jhon James Mora, M. E. Arias,

Biochemical and biochemical processes

Low molecular weight aromatic compounds released during the growth of several Streptomyces strains on wheat straw in solid-state fermentation (SSF) were identified by gas chromatography/mass spectrometry. The analysis of the compounds derived from p-hydroxyphenyl, guaiacyl and syringyl units (H, G and S respectively) of lignin indicate the ability of streptomycetes to depolymerize lignin from lignocellulose substrates. Differences in the relative area of the compounds obtained from fermented substrate from the uninoculated control were observed. Moreover, the different proportion of the aromatic compounds obtained from fermented wheat straw proved that the ability to depolymerize lignin in Streptomyces is strain specific. The predominance of acidic forms among these compounds showed an oxidative attack on lignin by Streptomyces. The high level of vanillic acid released from lignin by the strains Streptomyces UAH 23 and Streptomyces UAH 33 seems to indicate the breakdown of Cα-Cβ linkages in the lignin molecule. The biotechnological applications of ferulic and vanillic acid as precursors of food additives is also undertaken. © 1998 John Wiley & Sons, Ltd.