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Culturing Puccinia coronata on a Cell Monolayer of the Avena sativa Coleoptile

1983; Wiley; Volume: 108; Issue: 3-4 Linguagem: Inglês

10.1111/j.1439-0434.1983.tb00583.x

1439-0434

K. Mendgen, Elvira Dressler,

Fungal and yeast genetics research

Abstract A culture of Puccinia coronata on an epidermal cell monolayer dissected from the Avena sativa caleoptile is described. To induce fungal development, infection structure formation had to be induced with a heat treatment. The host cells were fed with 1 % sucrose (or glucose or manitol) to sustain fungal growth. Under these conditions, normal development of hyphae, haustoria with haustorial mother cells and uredospores occurred. Uredospores had normal infectivity.