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PiggyBac Transposon Mutagenesis: A Tool for Cancer Gene Discovery in Mice

2010; American Association for the Advancement of Science; Volume: 330; Issue: 6007 Linguagem: Inglês

10.1126/science.1193004

1095-9203

Roland Rad, Lena Rad, Wei Wang, Juan Cadiñanos, George S. Vassiliou, Stephen Rice, Lia S. Campos, Kosuke Yusa, Ruby Banerjee, Meng Amy Li, Jorge de la Rosa, Alexander Strong, Dong Lu, Peter Ellis, Nathalie Conte, Fengtang Yang, Pentao Liu, Allan Bradley,

RNA Interference and Gene Delivery

Piggybacking on Cancer Genes Transposons are mobile segments of DNA that can insert in or near important genes to cause mutations that disrupt gene function. Rad et al. (p. 1104 , published online 14 October) adapted a mutagenic transposon called Piggybac, originally derived from a moth, into a tool for discovery of cancer-causing genes in mice. Mobilization of Piggybac in mice was associated with the development of leukemias and solid tumors. In many instances the causative mutations, which were identified by mapping the Piggybac integration sites, were within genes not previously implicated in cancer.