Phytoene Desaturase: Genes, Enzymes and Phylogenetic Aspects
1994; Elsevier BV; Volume: 143; Issue: 4-5 Linguagem: Inglês
10.1016/s0176-1617(11)81805-5
ISSN1618-1328
Autores Tópico(s)Lipid metabolism and biosynthesis
ResumoRecent work on phytoene desaturases and also on ζ-carotene desaturase is presented and discussed. This involves functional complementation of phytoene desaturation by heterologous expression of different genes coding for this enzyme originating from Rhodobacter capsulatus, Erwinia uredovora, and Synechococcus and the enzymatic characterization of purified enzymes. The results obtained demonstrate that different phytoene desaturases exist with respect to the catalytic reaction and the protein sequence. The gene for the proposed ζ-carotene desaturase was cloned from the cyanobacterium Anabaena and the amino acid sequence deduced from the gene sequence compared to the other carotene desaturases. The phylogenetic relationship between different types of phytoene desaturases and the ζ-carotene desaturase is discussed.
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