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The Arrhenius plot behaviour of rat liver 5′-nucleotidase in different lipid environments

1978; Elsevier BV; Volume: 514; Issue: 2 Linguagem: Inglês

10.1016/0005-2736(78)90291-2

1879-2642

Keith K. Stanley, J. Paul Luzio,

Biochemical and Molecular Research

The plasma membrane enzyme 5′-nucleotidase (EC 3.1.3.5) was prepared from rat liver as a complex with sphingomyelin or in detergent-solubilized forms. Each preparation exhibited a Km and Arrhenius break temperature indistinguishable from that of 5′-nucleotidase in plasma membranes. Measurement of fatty acid profiles, cholesterol and phospholipid content however showed a very wide variation between these preparations. We conclude that the biphasic nature of the Arrhenius plot of 5′-nucleotidase may be a property of the enzyme rather than its lipid environment.