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DNA polymerase α is associated with replicating SV4O nucleoprotein complexes

1978; Oxford University Press; Volume: 5; Issue: 5 Linguagem: Inglês

10.1093/nar/5.5.1715

1362-4962

Bernd Otto, Ellen Fanning,

Bacteriophages and microbial interactions

Simian virus 40 (SV40) nucleoprotein complexes were extracted from nuclei of infected monkey cells and fractionated on neutral sucrose density gradients. Complexes which contai replicating SV40 DNA (95S) separated well from those containing closed circular supercoiled viral DNA (75S). DNA polymerase activity was associated with the replicating nucleoprotein complexes but not with the slower sedimenting complexes. This DNA polymerase activity coprecipitated with the nucleoprotein complexes in the presence of MgCl2 and remained associated with the 95S complexes. This DNA polymerase activity has been identified as primarily DNA polymerase α on the basis of its sedimentation behavior, optimum salt concentration, and sensitivity to N-ethylmaleimide. DNA polymerase γ activity was also detected in the complexes, but DNA polymerase β was not associated with the complexes.