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A receptor-mediated gene delivery system using streptavidin and biotin-derivatized, pegylated epidermal growth factor

2002; Elsevier BV; Volume: 83; Issue: 1 Linguagem: Inglês

10.1016/s0168-3659(02)00166-9

1873-4995

Haeshin Lee, Tae Hyoung Kim, Tae Gwan Park,

Advanced biosensing and bioanalysis techniques

An efficient receptor-mediated non-viral gene delivery formulation based on mono-pegylated recombinant human epidermal growth factor (EGF) was developed using a streptavidin–biotin system. Biotin-derivatized and mono-pegylated EGF was prepared by conjugating a biotin-PEG-NHS derivative to EGF and purified through a chromatographic method. Luciferase plasmid DNA and polyethylenimine (PEI) were complexed to form positively charged nanoparticles on which negatively charged streptavidin was first coated and then biotin-PEG-EGF conjugate was immobilized via streptavidin–biotin interaction. The EGF-PEG-biotin–streptavidin–PEI–DNA complexes were characterized in terms of their effective diameter and surface zeta (ζ)-potential value under various formulation conditions. The formulated complexes exhibited high transfection efficiency (∼108 in luciferase activity) with no inter-particle aggregation. This was attributed to enhanced cellular uptake of the resultant complexes via receptor-mediated endocytosis. Furthermore, in the presence of serum proteins, a slight decrease in transfection efficiency was observed due to the presence of PEG chains on the surface.