Enantioselective antiplatelet actions of nipecotamides
1993; Elsevier BV; Volume: 69; Issue: 4 Linguagem: Inglês
10.1016/0049-3848(93)90035-m
ISSN1879-2472
AutoresR. Gollamudi, Zixia Feng, Elwood O. Dillingham, S.E. Bond, Guangli Han, Somna R. Salgia,
Tópico(s)Chemical Synthesis and Analysis
ResumoTwo synthetic racemic nipecotamides, 1-decyl-3-(N,N-diethylcarbamoyl)piperidine hydrobromide (1) and α,α'-bis[3-(N-benzyl-N-methylcarbamoyl)piperidino]-p-xylene dihydrobromide (2) were resolved on a chiral α1-acid glycoprotein semipreparative HPLC column. Thus, rac.1 was resolved into two enantiomers 1A-(+) and 1B-(-); rac.2 was separated into the optical antipodes 2A-(-) and 2C-(+), and the meso diastereomer 2B-(0). Also on a preparative scale, 97% pure 2C was obtained via diastereomeric salt formation using dibenzoyl-L-(-)-tartaric acid. The individual isomers were evaluated for their platelet aggregation inhibitory potency. In inhibiting ADP-induced aggregation of human platelets in vitro, 1B-(-) was 4 times more potent than its optical antipode 1A-(+), and 2C-(+) was 6 times as active as 2A-(-); the meso diastereomer 2B-(0) had intermediate activity. With collagen as the agonist, 1B-(-) was twice as active as 1A-(+), and 2C-(+), the most active compound in this series (IC50= 0.96 μM), was 10 times more potent than its antipode 2A-(-). Again, the meso diastereomer 2B-(0) had intermediate activity. These results demonstrate the enantioselective antiplatelet actions of mono- and bis- nipecotamide derivatives.
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