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Rapid and multi-level characterization of trastuzumab using sheathless capillary electrophoresis-tandem mass spectrometry

2013; Landes Bioscience; Volume: 5; Issue: 3 Linguagem: Inglês

10.4161/mabs.23995

1942-0870

Rabah Gahoual, Alicia Burr, Jean‐Marc Busnel, Lauriane Kühn, Phillipe Hammann, Alain Beck, Yannis‐Nicolas François, Emmanuelle Leize‐Wagner,

Glycosylation and Glycoproteins Research

Monoclonal antibodies (mAbs) are highly complex proteins that display a wide range of microheterogeneity that requires multiple analytical methods for full structure assessment and quality control. As a consequence, the characterization of mAbs on different levels is particularly product - and time - consuming. This work presents the characterization of trastuzumab sequence using sheathless capillary electrophoresis (referred as CESI) – tandem mass spectrometry (CESI-MS/MS). Using this bottom-up proteomic-like approach, CESI-MS/MS provided 100% sequence coverage for both heavy and light chain via peptide fragment fingerprinting (PFF) identification. The result was accomplished in a single shot, corresponding to the analysis of 100 fmoles of digest. The same analysis also enabled precise characterization of the post-translational hot spots of trastuzumab, used as a representative widely marketed therapeutic mAb, including the structural confirmation of the five major N-glycoforms.