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Stability of cisplatin and its monohydrated complex in blood, plasma and ultrafiltrate — implications for quantitative analysis

1995; Elsevier BV; Volume: 13; Issue: 4-5 Linguagem: Inglês

10.1016/0731-7085(95)01292-s

1873-264X

Anita Andersson, Hans Ehrsson,

Acute Lymphoblastic Leukemia research

The stability of cisplatin and its monohydrated complex has been studied in blood, plasma and plasma ultrafiltrate at 37°C (pH 7.4). Intact cisplatin and the monohydrated complex were determined by liquid chromatography with post-column derivatization. The half lives for cisplatin and the monohydrated complex were 1.43 ± 0.03 h (SEM) and 0.36 ± 0.03 h (SEM), respectively, in blood and 0.88 ± 0.05 h (SEM) and 0.26 ± 0.02 h (SEM), respectively, in plasma. The compounds were unstable at −25°C (t12 for cisplatin was 52 ± 5 h (SEM) and for the monohydrated compound 26 ± 2 h (SEM)), but at −70°C both compounds were stable for at least 3 weeks. The monohydrated complex was found to be formed to a small extent when cisplatin was added to plasma (37°C, pH 7.4). A sampling procedure using centripetal ultrafiltration of whole blood was evaluated and found applicable if the samples were stored at 0°C and ultracentrifuged within 1 h.