The reconstitution of histone H 3 H 4 tetramer from acid extracted histones in the absence of urea
1982; Wiley; Volume: 145; Issue: 1 Linguagem: Inglês
10.1016/0014-5793(82)81221-0
ISSN1873-3468
AutoresGeorge G. Lindsey, Patricia Thompson, L.R. Purves, Claus von Holt,
Tópico(s)Spectroscopy and Quantum Chemical Studies
ResumoSimple mixing of acid purified histones H 3 and H 4 in equimolar quantities at low ionic strength near pH 7 does not yield the tetramer but rather a high M r aggregate. Dialysis of acid extracted total or core histones into 2 M NaCl 150 mM phosphate (pH 7.4) followed by fractionation of the histone complexes at lower ionic strength (150 mM NaCl) results in an H 3 H 4 tetramer of a structure identical to that derived from salt‐extracted histones. Dialysis of acid extracted total or core histones directly into the lower ionic strength buffer with subsequent fractionation, results in H 3 H 4 tetramer of closely similar structure.
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