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High-throughput screening as a tool for agrochemical discovery: automated synthesis, compound input, assay design and process management

1998; Wiley; Volume: 54; Issue: 4 Linguagem: Inglês

10.1002/(sici)1096-9063(199812)54

1096-9063

Stuart M. Ridley, A. Clare Elliott, Mabel Yeung, David Youle,

Microbial Metabolic Engineering and Bioproduction

Pesticide ScienceVolume 54, Issue 4 p. 327-337 PaperFull Access High-throughput screening as a tool for agrochemical discovery: automated synthesis, compound input, assay design and process management† Stuart M. Ridley, Corresponding Author Stuart M. Ridley [email protected] Zeneca Agrochemicals, Jealott's Hill Research Station, Bracknell, Berkshire RG42 6EY, UKZeneca Agrochemicals, Jealott's Hill Research Station, Bracknell, Berkshire RG42 6EY, UKSearch for more papers by this authorA. Clare Elliott, A. Clare Elliott Zeneca Agrochemicals, Jealott's Hill Research Station, Bracknell, Berkshire RG42 6EY, UKSearch for more papers by this authorMabel Yeung, Mabel Yeung Zeneca Agrochemicals, Jealott's Hill Research Station, Bracknell, Berkshire RG42 6EY, UKSearch for more papers by this authorDavid Youle, David Youle Zeneca Agrochemicals, Jealott's Hill Research Station, Bracknell, Berkshire RG42 6EY, UKSearch for more papers by this author Stuart M. Ridley, Corresponding Author Stuart M. Ridley [email protected] Zeneca Agrochemicals, Jealott's Hill Research Station, Bracknell, Berkshire RG42 6EY, UKZeneca Agrochemicals, Jealott's Hill Research Station, Bracknell, Berkshire RG42 6EY, UKSearch for more papers by this authorA. Clare Elliott, A. Clare Elliott Zeneca Agrochemicals, Jealott's Hill Research Station, Bracknell, Berkshire RG42 6EY, UKSearch for more papers by this authorMabel Yeung, Mabel Yeung Zeneca Agrochemicals, Jealott's Hill Research Station, Bracknell, Berkshire RG42 6EY, UKSearch for more papers by this authorDavid Youle, David Youle Zeneca Agrochemicals, Jealott's Hill Research Station, Bracknell, Berkshire RG42 6EY, UKSearch for more papers by this author First published: 26 March 1999 https://doi.org/10.1002/(SICI)1096-9063(199812)54:4 3.0.CO;2-CCitations: 23 † One of a collection of papers on various aspects of agrochemicals research contributed by staff and collaborators of Zeneca Agrochemicals UK and Zeneca Ag Products USA. The papers were collected and collated by Dr B. C. Baldwin and Dr D. Tapolczay. AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onEmailFacebookTwitterLinkedInRedditWechat Abstract High-throughput screening (HTS) is becoming increasingly important within the agrochemical industry as a means of sustaining and improving the flow of novel products to the marketplace. Technology changes within agrochemical research have to some extent followed progress in the pharmaceutical industry in adopting in-vitro-based HTS strategies for lead discovery. However, agrochemical invention faces the unique challenge of combining the opportunities made available by HTS technology with the key advantage of being able to work in the laboratory with real, commercial, whole-organism targets. This paper describes approaches taken within Zeneca to harness these opportunities effectively through development of novel assay procedures for agrochemical discovery. Quality and quantity of chemical input to the HTS process is of paramount importance for the successful discovery of leads. Approaches employing the application of automated chemical synthesis to achieve this aim are reviewed. Adoption of a high-throughput screening strategy requires a fundamental change in areas such as process management, data manipulation and data analysis. Some of our experiences to date in managing these issues are described. © 1998 Society of Chemical Industry References 1 Main, B. G. & Rudge, D. A., Automated chemical synthesis. In International Symposium on Laboratory Automation and Robotics, ed. J. R. Strimaitis & G. L. Hawk. Zymark Corp., Hopkinton, MA, 01748-1668 USA, 1994 pp. 425–34. 2 Walter, G. C., Elliott, C. & Higgins, K. F., Internet http://www.islar.com/ABSTR97/Walter.htm. 3 Furka, A., Sebestyen, F., Asgedom, M. & Dibo, G., General method for rapid synthesis of multicomponent peptide mixtures. Int. J. Pept. Protein Res., 37 (1991) 487–93. 4 Hess, H. H. & Durr, J. E., Assay of inorganic and organic phosphorus in the 0.1–5 nanomole range. Anal. Biochem., 63 (1975) 607–13. 5 Howard, J. A. & Ridley, S. M., Acetyl-CoA carboxylase: a rapid novel assay procedure used in conjunction with the preparation of enzyme from maize leaves. FEBS Lett., 261 (1990) 261–4. 6 Turner, J. C. & Hall, N. P., Identification of genetically induced lesions and the sites of action of inhibitors affecting photorespiration by simple tests on leaf discs. J. Exp. Bot., 39 (1988) 345–51. 7 Hawkes, T. R., Cox, J. M., Barnes, N. J., Beautement, L. S., Edwards, L. S., Kipps, M. R., Langford, M. P., Lewis, T., Ridley, S. M. & Thomas, P. G., Imidazole glycerol phosphate dehydratase: a herbicide target. Proc. Brighton Crop Prot. Conf—Weeds (1993) 739–744. 8 Fersht, A. R., Editing mechanisms in protein synthesis. Rejection of valine by isoleucyl-tRNA synthetase. Biochemistry, 16 (1977) 1025–30. 9 Dixon, G., Scanlon, D., Casper, S. & Broad, P., A receptor gene assay for fungal sterol biosynthesis inhibitors. J. Steroid Biochem. Mol. Biol., 62 (1997) 165–71. 10 Nicolaou, K. C., Xiao, X.-Y., Parandoosh, Z., Senyei, A. & Nova, M. P., Radiofrequency encoded combinatorial chemistry. Angew. Chem., Int. Edn Engl., 34 (1995) 2289–91. 11 Internet http://www.irori.com 12 Xiao, X.-Y., Zhao, C., Potash, H. & Nova, M. P., Combinatorial chemistry with laser optical encoding. Angew. Chem., Int. Edn Engl., 36 (1997) 780–2. Citing Literature Volume54, Issue4Special Issue: Current research at Zeneca AgrochemicalsDecember 1998Pages 327-337 ReferencesRelatedInformation