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A real-time PCR assay for the specific identification of serotype O:9 of Yersinia enterocolitica

2005; Elsevier BV; Volume: 63; Issue: 2 Linguagem: Inglês

10.1016/j.mimet.2005.03.003

1872-8359

Nicklas Raun Jacobsen, Tatiana Bogdanovich, Mikael Skurnik, Peter Stephensen Lübeck, Peter Ahrens, Jeffrey Hoorfar,

Pharmacological Effects of Natural Compounds

A real-time PCR assay was developed based on a 181-bp fragment of the recently cloned per gene, including an internal amplification control (124 bp), for the detection of Yersinia enterocolitica O:9 (Ye O:9). The validation included 48 Ye O:9, 33 Y. enterocolitica non-O:9 and 35 other closely-related bacterial strains, containing per gene homologies. The assay was specific for the Ye O:9 tested, the detection limit was 1–10 genome copies of purified DNA and amplification efficiency was between 90.5–103%, indicating a linear regression throughout the detection window.