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Evaluation Of The Antimicrobial Effect Of Er:YAG Laser Irradiation Versus 1% Sodium Hypochlorite Irrigation For Root Canal Disinfection

2004; Wiley; Volume: 30; Issue: 1 Linguagem: Inglês

10.1111/j.1747-4477.2004.tb00162.x

1747-4477

Fernanda M. Perin, Suzelei de Castro França, Yara Teresinha Corrêa Silva‐Sousa, Edson Alfredo, Paulo César Saquy, Carlos Estrela, Manoel Damião Sousa‐Neto,

Dental Trauma and Treatments

This laboratory study evaluated Er:YAG laser antibacterial action in infected root canals. Forty-eight maxillary central incisors were used. After canal preparation, the teeth were autoclaved and divided into four groups: (1) non-treated teeth (control group); (2) teeth treated with NaOCl; (3) teeth irradiated with Er:YAG laser (7 Hz, 100 mJ, 80 pulses/canal, 11 sec) to the working length; (4) teeth irradiated similarly to, but 3 mm short, of the apex. The root canals from Groups 2, 3 and 4 were inoculated with 4 bacteria: Bacillus subtillus, Enterococcus faecalis, Pseudomonas aeruginosa, and Staphylococcus aureus, together with Candida albicans, and maintained for 24 h at 37 degrees C. All suspensions were adjusted to tube 2 of the MacFarland scale. The intracanal material was then collected with sterile paper points, which were placed in the canals for 5 min and then immersed in 5 ml of BHI medium. This was then seeded onto agar and stained by Gram's method. The NaOCl solutions and the Er:YAG laser irradiation to working length were effective against all five micro-organisms; however, 70% of the specimens irradiated 3 mm short of the apex remained infected.