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BIBTEX RIS

Determinação de etanol e voláteis relacionados em sangue e fluido oral por microextração em fase sólida em headspace associada à cromatografia gasosa com detector de ionização em chama

2009; Brazilian Chemical Society; Volume: 32; Issue: 9 Linguagem: Inglês

10.1590/s0100-40422009000900031

ISSN

1678-7064

Autores

Lílian de Lima Feltraco, Marina Venzon Antunes, Rafael Linden,

Tópico(s)

Analytical Methods in Pharmaceuticals

Resumo

FLAME-IONIZATION DETECTOR.The aim of this study was to validate a method for the determination of acethaldehyde, methanol, ethanol, acetone and isopropanol employing solid-phase microextraction associated to gas chromatography with flame ionization detection.The operational conditions of SPME were optimized by response surface analysis.The calibration curves for all compounds were linear with r 2 > 0.9973.Accuracy (89.1-109.0%),intra-assay precision (1.8-8.5%) and inter-assay precision (2.2-8.2%) were acceptable.The quantification limit was 50 µg/mL.The method was applied to the meaurement of ethanol in blood and oral fluid of a group of volunteers.Oral fluid ethanol concentrations were not directly correlated with blood concentrations.

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