Enantioselective synthesis of pure (R,R)-2,3-butanediol in Escherichia coli with stereospecific secondary alcohol dehydrogenases
2009; Royal Society of Chemistry; Volume: 7; Issue: 19 Linguagem: Inglês
10.1039/b913501d
ISSN1477-0539
AutoresYajun Yan, Chia-Chi Lee, James C. Liao,
Tópico(s)Innovative Microfluidic and Catalytic Techniques Innovation
ResumoWe characterized the activity and stereospecificity of four secondary alcohol dehydrogenases (sADHs) towards acetoin reduction and constructed synthetic pathways in E. coli to produce enantiomerically pure (R,R)-2,3-butanediol (2,3-BDO) from glucose with a titer of 6.1 g/L (enantio purity >99%), and yield of 0.31 g product/g glucose (62% of theoretical maximum).
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