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The fate of bovine bone marrow stromal cells in hydrogels: a comparison to nucleus pulposus cells and articular chondrocytes

2009; Wiley; Volume: 3; Issue: 4 Linguagem: Inglês

10.1002/term.167

1932-7005

Stephan Zeiter, Marije van der Werf, Keita Ito,

Proteoglycans and glycosaminoglycans research

Journal of Tissue Engineering and Regenerative MedicineVolume 3, Issue 4 p. 310-320 Research Article The fate of bovine bone marrow stromal cells in hydrogels: a comparison to nucleus pulposus cells and articular chondrocytes Stephan Zeiter, Stephan Zeiter AO Research Institute, Davos, Switzerland These authors contributed equally to this study.Search for more papers by this authorMarije van der Werf, Marije van der Werf AO Research Institute, Davos, Switzerland Department of Biomedical Engineering, Eindhoven University of Technology, The Netherlands These authors contributed equally to this study.Search for more papers by this authorKeita Ito, Corresponding Author Keita Ito k.ito@tue.nl AO Research Institute, Davos, Switzerland Department of Biomedical Engineering, Eindhoven University of Technology, The NetherlandsDepartment of Biomedical Engineering, Eindhoven University of Technology, PO Box 513, WH 3.115, 5600 MB Eindhoven, The Netherlands.Search for more papers by this author Stephan Zeiter, Stephan Zeiter AO Research Institute, Davos, Switzerland These authors contributed equally to this study.Search for more papers by this authorMarije van der Werf, Marije van der Werf AO Research Institute, Davos, Switzerland Department of Biomedical Engineering, Eindhoven University of Technology, The Netherlands These authors contributed equally to this study.Search for more papers by this authorKeita Ito, Corresponding Author Keita Ito k.ito@tue.nl AO Research Institute, Davos, Switzerland Department of Biomedical Engineering, Eindhoven University of Technology, The NetherlandsDepartment of Biomedical Engineering, Eindhoven University of Technology, PO Box 513, WH 3.115, 5600 MB Eindhoven, The Netherlands.Search for more papers by this author First published: 24 March 2009 https://doi.org/10.1002/term.167Citations: 17 AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Bone marrow-derived stromal cells (BMSCs) are good candidates for cell-based tissue regeneration. For such purposes, cell survival within three-dimensional (3D) scaffolds is often desirable. We hypothesize that undifferentiated BMSCs will have difficulties thriving within these gels, in contrast to articular chondrocytes (ACs) and nucleus pulposus cells (NPCs), but that early chondrogenic differentiation of the former will increase their survival. BMSCs, ACs and NPCs cast in 1.2% alginate or 2% agarose were cultured for 21 days in serum-containing media. BMSCs were also cultured in medium with 10 ng/ml TGF-β1. By day 21, NPCs and ACs proliferated, maintained upregulation of aggrecan and collagen type II, produced glycosaminoglycans and stained positively for collagen type II in both scaffolds. In contrast, the number of living BMSCs and the DNA content of their constructs decreased in both scaffolds. Addition of TGF-β1 resulted in cell survival and behaviour more similar (gene expression, glycosaminoglycan production and collagen type II synthesis) to ACs and NPCs. This study demonstrated that, unlike ACs and NPCs, undifferentiated BMSCs have more difficulty thriving within hydrogels, but that this can be improved by chondrogenic induction. Hence, immediate conditioning of BMSCs could be a worthwhile strategy. Copyright © 2009 John Wiley & Sons, Ltd. Citing Literature Volume3, Issue4June 2009Pages 310-320 RelatedInformation