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Decellularization and Recellularization Technologies in Tissue Engineering

2014; SAGE Publishing; Volume: 23; Issue: 4-5 Linguagem: Inglês

10.3727/096368914x678382

1555-3892

Ru‐Huei Fu, Yu-Chi Wang, Shih‐Ping Liu, Ton-Ru Shih, Hsin-Lien Lin, Yue-Mi Chen, Jiun-Huei Sung, Chia-Hui Lu, Jing-Rong Wei, Zih-Wan Wang, Shyh-Jer Huang, Chang‐Hai Tsai, Woei‐Cherng Shyu, Shinn‐Zong Lin,

3D Printing in Biomedical Research

Decellularization is the process by which cells are discharged from tissues/organs, but all of the essential cues for cell preservation and homeostasis are retained in a three-dimensional structure of the organ and its extracellular matrix components. During tissue decellularization, maintenance of the native ultrastructure and composition of the extracellular matrix (ECM) is extremely acceptable. For recellularization, the scaffold/matrix is seeded with cells, the final goal being to form a practical organ. In this review, we focus on the biological properties of the ECM that remains when a variety of decellularization methods are used, comparing recellularization technologies, including bioreactor expansion for perfusion-based bioartificial organs, and we discuss cell sources. In the future, decellularization–recellularization procedures may solve the problem of organ assembly on demand.