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Effects of pH value and substrate concentration on hydrogen production from the anaerobic fermentation of glucose

2008; Elsevier BV; Volume: 33; Issue: 24 Linguagem: Inglês

10.1016/j.ijhydene.2008.09.048

1879-3487

Zhi Li, Hui Wang, Zihan Tang, Xiukang Wang, Jie Bai,

Microbial Fuel Cells and Bioremediation

A series of batch experiments were conducted to investigate the effects of pH and glucose concentrations on biological hydrogen production by using the natural sludge obtained from the bed of a local river as inoculant. Batch experiments numbered series I and II were designed at an initial and constant pH of 5.0–7.0 with 1.0 increment and four different glucose concentrations (5.0, 7.5, 10 and 20 g glucose/L). The results showed that the optimal condition for anaerobic fermentative hydrogen production is 7.5 g glucose/L and constant pH 6.0 with a maximum H2 production rate of 0.22 mol H2 mol−1 glucose h−1, a cumulative H2 yield of 1.83 mol H2 mol−1 glucose and a H2 percentage of 63 in biogas.