Portal de Periódicos da CAPES

Importance of size-to-charge ratio in construction of stable and uniform nanoscale RNA/dendrimer complexes

2007; Royal Society of Chemistry; Volume: 5; Issue: 22 Linguagem: Inglês

10.1039/b711242d

1477-0539

Xin‐Cheng Shen, Jiehua Zhou, Xiaoxuan Liu, Jiangyu Wu, Fanqi Qu, Zhi-Ling Zhang, Dai-Wen Pang, Gilles Quéléver, Cheng‐Cai Zhang, Ling Peng,

Advanced biosensing and bioanalysis techniques

Formation of RNA/dendrimer complexes between various RNA molecules and PAMAM dendrimers was studied using atomic force microscopy. Our results demonstrate that effective construction of stable nanoscale and uniform RNA/dendrimer complexes depends critically on the size of the RNA molecule, the dendrimer generation and the charge ratio between the dendrimer and the RNA. Larger RNA molecules, higher generations of dendrimers and larger dendrimer-to-RNA charge ratios lead to the formation of stable, uniform nanoscale RNA/dendrimer complexes. These findings provide new insights in developing dendrimer systems for RNA delivery.