Arabidopsis Argonaute10 Specifically Sequesters miR166/165 to Regulate Shoot Apical Meristem Development
2011; Cell Press; Volume: 145; Issue: 2 Linguagem: Inglês
10.1016/j.cell.2011.03.024
ISSN1097-4172
AutoresHongliang Zhu, Fuqu Hu, Ronghui Wang, Xin Zhou, Sing‐Hoi Sze, Lisa Wen Liou, A. C. Barefoot, Martin B. Dickman, Xiuren Zhang,
Tópico(s)Plant nutrient uptake and metabolism
ResumoThe shoot apical meristem (SAM) comprises a group of undifferentiated cells that divide to maintain the plant meristem and also give rise to all shoot organs. SAM fate is specified by class III HOMEODOMAIN-LEUCINE ZIPPER (HD-ZIP III) transcription factors, which are targets of miR166/165. In Arabidopsis, AGO10 is a critical regulator of SAM maintenance, and here we demonstrate that AGO10 specifically interacts with miR166/165. The association is determined by a distinct structure of the miR166/165 duplex. Deficient loading of miR166 into AGO10 results in a defective SAM. Notably, the miRNA-binding ability of AGO10, but not its catalytic activity, is required for SAM development, and AGO10 has a higher binding affinity for miR166 than does AGO1, a principal contributor to miRNA-mediated silencing. We propose that AGO10 functions as a decoy for miR166/165 to maintain the SAM, preventing their incorporation into AGO1 complexes and the subsequent repression of HD-ZIP III gene expression.PaperFlickeyJraWQiOiI4ZjUxYWNhY2IzYjhiNjNlNzFlYmIzYWFmYTU5NmZmYyIsImFsZyI6IlJTMjU2In0.eyJzdWIiOiI3NWEzYzkxMjMwOWUwMDVjZThlMmRmYWIyZmYyNGU3OCIsImtpZCI6IjhmNTFhY2FjYjNiOGI2M2U3MWViYjNhYWZhNTk2ZmZjIiwiZXhwIjoxNjc5MTM2MTQxfQ.ZmES55FME8OCAzXOMRdfoNWwNAkd0pRZ1ScmpaabF_cWSuCbqXrCBPfJFSMtAJMAY_WVwW4cxEXEz3JS4zzWq5CqZ9GG9ZrA8OgUC8cBs5dGS2QvX5Ov60aSWDeanpgVnUBb4UjU7GgyJCbDah_BJvb1GF0lI2dY_ZmMuNV3omjAPBUqfz8chjNC4N183arWtQ4Mwdh0HbdMD6Lm1QKlZ9B5_dHgouQJe8nvQQAinMtFVGMnjCV09-yw5H1sjrqi5n22u5ymDsfeLJyfaK5aP07HIKRaExAwmc45wayu1R4jtqrM42FAEnO0i-aOiFH0_EVBU2DN0rv7xQwvCF6Cog(mp4, (8.8 MB) Download video
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