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Artigo Revisado por pares
BIBTEX RIS

Quantitative analysis of bacterial medium-chain-length poly([R]-3-hydroxyalkanoates) by gas chromatography

2007; Elsevier BV; Volume: 1143; Issue: 1-2 Linguagem: Inglês

10.1016/j.chroma.2007.01.002

ISSN

1873-3778

Autores

Patrick Furrer, Roland Hany, Daniel Rentsch, Andreas Grubelnik, Katinka Ruth, Sven Panke, Manfred Zinn,

Tópico(s)

Microplastics and Plastic Pollution

Resumo

Standard chromatographic methods for the quantification of bacterial poly(3-hydroxyalkanoate) (PHA) proved to be inappropriate for the analysis of medium-chain-length PHA (mcl-PHA). Transesterification catalyzed by protic acids is not quantitative for mcl-PHA under common conditions due to slow reaction kinetics and formation of side-products in case of functionalized side-chains. To circumvent these limitations, an improved method for the quantification of mcl-PHA by GC-FID was developed. Boron trifluoride in methanol was successfully applied to quantitatively methanolyse different mcl-PHA (recovery >94%). This novel method is well-suited for the analysis of purified mcl-PHA as well as for mcl-PHA in biomass.

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