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Pharmacokinetics and tissue residues of marbofloxacin in crucian carp ( Carassius auratus ) after oral administration

2009; Wiley; Volume: 40; Issue: 6 Linguagem: Inglês

10.1111/j.1365-2109.2008.02146.x

1365-2109

Yanlei Zhu, Yeping Tan, Chunmei Wang, Nan Zhang, Yunping Liu, Lina Liu, Chunming Li, Xiaocong Lu, Jiyue Cao,

Bacterial Identification and Susceptibility Testing

Pharmacokinetics and residue elimination of marbofloxacin (MBF) were studied in crucian carp (Carassius auratus, 250±30 g) kept at two water temperatures of 15 and 25 °C. Marbofloxacin concentrations in plasma and tissues were analysed by means of high-performance liquid chromatography using an ultraviolet detector. The limits of detection were 0.02 μg mL−1, 0.02 μg g−1, 0.025 μg g−1, 0.02 μg g−1 and 0.025 μg g−1 in plasma and muscle, skin, liver and kidney respectively. Fish were administered orally at a single dosage of 10 mg kg−1 body weight in the PK group. The data were fitted to two-compartment open models at both temperatures. At 15 °C, the absorption half-life () and distribution half-life (t1/2α) of the drug were 0.36 and 4.48 h respectively. The corresponding values at 25 °C were 0.23 and 0.87 h respectively. The elimination half-life (t1/2β) was 50.75 h at 15 °C and 25.05 h at 25 °C. The maximum MBF concentration (Cmax) differed little between 15 (6.43 μg mL−1) and 25 °C (8.36 μg mL−1). The time to peak concentration was 1.74 h at 15 °C and 0.78 h at 25 °C. The apparent volume of distribution (Vd/F) of MBF was estimated to be 1.36 and 0.87 L kg−1 at 15 and 25 °C respectively. The area under the concentration–time curve (AUC) was 301.80 μg mL−1 h at 15 °C and 182.80 μg mL−1 h at 25 °C. The total clearance of MBF was computed as 0.03 and 0.05 L h−1 kg−1 at 15 and 25 °C respectively. After repeated oral administration at a dosage of 10 mg kg−1 body weight per day for 3 days, the results showed that the elimination half-lives () of MBF from all tissues at 15 °C were longer than that at 25 °C. Therefore, water temperature is an important factor to be considered when deciding a reasonable withdrawal time.