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Developmental regulation of GAP-43, glutamine synthetase and β-actin mRNA in rat cortical astrocytes

1991; Elsevier BV; Volume: 64; Issue: 1-2 Linguagem: Inglês

10.1016/0165-3806(91)90228-b

1872-6755

Anna da Cunha, Vincent J. Aloyo, Ljubiša Vitković,

interferon and immune responses

Steady-state levels of mRNA encoding growth-associated protein 43 (GAP-43), glutamine synthetase (GS) and beta-actin were measured during development of neonatal rat cortical astrocytes in primary culture. GAP-43 mRNA and protein decreased rapidly during the first 2 weeks and slowly thereafter. In contrast, GS mRNA increased approximately 3-fold during the first 2 weeks and reached maximum by day 15. Actin mRNA first increased up to 8 days and decreased thereafter reaching a constant amount of 15 days, similar to the initial low value. Thus, GAP-43, GS and beta-actin mRNA levels are differentially regulated during development of astrocytes in primary culture. Because the patterns of expression of astrocytic markers GS and GFAP (shown previously) in vitro and in vivo are similar to each other, primary cultures of astrocytes may be an excellent system for investigating mechanisms of developmental regulation of these genes.