Produção Nacional
Revisado por pares
Supercritical fluid extraction of hernandulcin from Lippia dulcis Trev.
2011; Elsevier BV; Volume: 63; Linguagem: Inglês
10.1016/j.supflu.2011.12.003
ISSN1872-8162
AutoresPatricia Francisco de Oliveira, Ricardo Antônio Francisco Machado, Ariovaldo Bolzan, Danielle Barth,
Tópico(s)Essential Oils and Antimicrobial Activity
ResumoThis paper presents the results of the supercritical fluid extraction (SFE) of Lippia dulcis, which has not yet been researched for this plant matrix. The SFE experiments were performed in a laboratory-scale pilot unit. Hydrodistillation was adopted as a method for comparison. Chromatographic analyses (GCMS, LCMS and HPLC) were performed to evaluate the composition of the extracts, indicating the presence of hernandulcin, a sesquiterpene of sweet flavor, and also of other compounds. The results indicated higher total extract yields in the SFE process (from 1.7 to 3.4%), for all parameters tested, in comparison to hydrodistillation (0.6%). The conditions of 14 MPa and 40 °C promoted the highest yield in the SFE process, but due to qualitative aspects of the extracts 12 MPa and 35 °C were selected as the most appropriate conditions for the SFE of L. dulcis. Qualitative GCMS results for the SFE extracts and hydrodistilled essential oil showed good agreement with the literature. The percentage of hernandulcin in the extracts obtained by supercritical fluid extraction (12 MPa/35 °C, 2.6 ± 0.1% total yield) was 41.9 ± 0.01% (mass of hernandulcin relative to the initial mass of extract in the sample).
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