Double minute chromosomes are not centromeric regions of the host chromosomes
2009; BioMed Central; Volume: 83; Issue: 1 Linguagem: Inglês
10.1111/j.1601-5223.1976.tb01573.x
ISSN1601-5223
AutoresGöran Levan, Nils Mandahl, U Bregula, George Klein, ALBERT LEVAN,
Tópico(s)RNA and protein synthesis mechanisms
ResumoThe SEWA ascites tumor of the mouse contains, in addition to the ordinary chromosomes, a varying number of minute chromatin bodies, called double minutes (dms). Among 394 cells, 43 were without dms, the others had from 1 to 300 dms. The appearance of the dms suggested that they were acentric, which was also supported by the finding that even the largest dms, which were spheres with a diameter larger than the ordinary chromatid breadth, exhibited no C-heterochromatin in Hoechst 33258 fluorescence nor in C-banding with the BSG technique. The conclusion was that the dms of the SEWA tumor have not originated from centromeric regions of ordinary mouse chromosomes.
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