Direct Electrochemiluminescence Detection of Oxidized DNA in Ultrathin Films Containing [Os(bpy) 2 (PVP) 10 ] 2+
2004; American Chemical Society; Volume: 126; Issue: 28 Linguagem: Inglês
10.1021/ja048615+
ISSN1943-2984
AutoresLynn Dennany, Robert J. Forster, Blánaid White, Malcolm R. Smyth, James F. Rusling,
Tópico(s)Electrochemical Analysis and Applications
ResumoDirect electrochemiluminescence (ECL) involving oxidized DNA was demonstrated in ultrathin films of cationic polymer [Os(bpy)2(PVP)10]2+ [PVP = poly(vinyl pyridine)] assembled layer-by-layer with DNA or oligonucleotides. Electrochemically oxidized Os(II) sites generated ECL from films containing oxo-guanines on DNA formed by chemical oxidation using Fenton reagent. Films combining DNA, [Ru(bpy)2(PVP)10]2+, and [Os(bpy)2(PVP)10]2+ had Os(II) sites that produced ECL specific for oxidized DNA, and Ru(II) sites gave ECL from reaction with oxo-adenines, chemically damaged DNA, and possibly from cleaved DNA strands.
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