NMR Solution Structure of Viscotoxin C1 from Viscum Album Species Coloratum ohwi : Toward a Structure−Function Analysis of Viscotoxins
2003; American Chemical Society; Volume: 42; Issue: 43 Linguagem: Inglês
10.1021/bi034762t
ISSN1943-295X
AutoresSilvia Romagnoli, Federico Fogolari, Maddalena Catalano, Lucia Zetta, Gerhard Schäller, Konrad Urech, Matteo Giannattasio, Laura Ragona, Henriette Molinari,
Tópico(s)Botanical Research and Chemistry
ResumoThe high resolution three-dimensional structure of the newly discovered plant viscotoxin C1, from the Asiatic Viscum album ssp. Coloratum ohwi, has been determined in solution by 1H NMR spectroscopy at pH 3.6 and 285 K. The viscotoxin C1-fold, consisting of a helix−turn−helix motif and a short stretch of an antiparralel β-sheet is very similar to that found for the highly similar viscotoxins A2 and A3 and for other related thionins. Different functional properties of members of the thionin family are discussed here in light of the structural and electrostatic properties. Among the very homologous family of α- and β-thionins, known for their antimicrobial activity, the viscotoxin subfamily differs from the other members because of its high toxicity against tumoral cells. Key residues for the modulation of viscotoxin cytotoxicity have been identified on the basis of sequence and structural alignment.
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