Portal de Periódicos da CAPES

ADRENALINE STIMULATES CHOLESTEROL SIDE-CHAIN CLEAVAGE CYTOCHROME P450 mRNA ACCUMULATION IN BOVINE ADRENOCORTICAL CELLS

1991; Bioscientifica; Volume: 131; Issue: 2 Linguagem: Inglês

10.1677/joe.0.131r005

1479-6805

Monika Ehrhart‐Bornstein, SR Bornstein, W.H. Trzeclak, Henning Usadel, H. Güse‐Behling, Michael R. Waterman, Werner A. Scherbaum,

Pharmacological Effects and Assays

ABSTRACT The effect of adrenaline on the accumulation of mRNA encoding cholesterol side-chain cleavage cytochrome P450 (P450 scc ) and cortisol secretion was studied in bovine adrenocortical cells in primary culture. Treatment of cultured cells with adrenaline resulted in a 2-fold increase in mRNA encoding P-450 scc , as revealed by Northern blot analysis. Under these conditions the maximal stimulation with ACTH resulted in a 6-fold accumulation of mRNA encoding P450 scc . The effect of adrenaline on the expression of P450 scc was abolished by the ß-blocker propranolol, while propranolol had no effect on ACTH-induced P450 scc mRNA accumulation. Adrenaline stimulated the secretion of cortisol in a dose-dependent manner with a median effective dose of 0.5 μmol/l. The adrenaline-stimulated cortisol secretion amounted to 42% of the effect of ACTH (0.1 nmol/l). Upon adrenaline treatment, cAMP concentration in the culture medium increased about 50-fold over the basal value. It is concluded that the stimulatory action of adrenaline upon cortisol formation requires ß-adrenergic receptors and is due, at least in part, to a cAMP-mediated increase in the accumulation of mRNA encoding P450 scc .