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Two KaiA‐binding domains of cyanobacterial circadian clock protein KaiC

2001; Wiley; Volume: 496; Issue: 2-3 Linguagem: Inglês

10.1016/s0014-5793(01)02408-5

1873-3468

Yasuhito Taniguchi, Akihiro Yamaguchi, Atsushi Hijikata, Hideo Iwasaki, Kyoko Kamagata, Masahiro Ishiura, Mitiko Gō, Takao Kondo,

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kaiABC , a gene cluster, encodes KaiA, KaiB and KaiC proteins that are essential to circadian rhythms in the unicellular cyanobacterium Synechococcus sp. strain PCC 7942. Kai proteins can interact with each other in all possible combinations. This study identified two KaiA‐binding domains (C KABD1 and C KABD2 ) in KaiC at corresponding regions of its duplicated structure. Clock mutations on the two domains and kaiA altered the strength of C KABD –KaiA interactions assayed by the yeast two‐hybrid system. Thus, interaction between KaiA and KaiC through C KABD1 and C KABD2 is likely important for circadian timing in the cyanobacterium.