Construction and Characterization of a Partial Library of Yeast Artificial Chromosomes from Human Chromosome 21
1991; Mary Ann Liebert, Inc.; Volume: 10; Issue: 4 Linguagem: Inglês
10.1089/dna.1991.10.301
ISSN1557-7430
AutoresM. Bellis, Audrey Gérard, J.P. Charlieu, Bertrand Marçais, Marie-Elisabeth Brun, E. Viégas-Pèquignot, D.A. CARTER, Gérard Roizès,
Tópico(s)Biofuel production and bioconversion
ResumoWe report a protocol for cloning large DNA fragments in yeast artificial chromosomes (YAC). A partial library has been constructed from a somatic hybrid containing chromosome 21 as the single source of human DNA. About 4.0 Mb of human DNA was recovered in 17 YAC clones. Three clones were analyzed by in situ hybridization and mapped on chromosome 21. One clone hybridized with the chromosome 21 centromeric region and may provide new insight both on the molecular structure of centromere and on the localization of Alzheimer disease gene.
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