RNA polymerase I-mediated expression of viral RNA for the rescue of infectious virulent and avirulent Rift Valley fever viruses
2008; Elsevier BV; Volume: 378; Issue: 2 Linguagem: Inglês
10.1016/j.virol.2008.05.033
ISSN1096-0341
AutoresAgnès Billecocq, Nicolas Gauliard, Nicolas Le May, Richard M. Elliott, Ramon Flick, Michèle Bouloy,
Tópico(s)Mosquito-borne diseases and control
ResumoRift Valley fever virus (RVFV, Bunyaviridae, Phlebovirus) is a mosquito-transmitted arbovirus that causes human and animal diseases in sub-Saharan Africa and was introduced into the Arabian Peninsula in 2000. Here, we describe a method of reverse genetics to recover infectious RVFV from transfected plasmids based on the use of the cellular RNA polymerase I promoter to synthesize viral transcripts. We compared its efficiency with a system using T7 RNA polymerase and found that both are equally efficient for the rescue of RVFV generating titers of approx. 10(7) to 10(8) pfu/ml. We used the RNA polymerase I-based system to rescue both attenuated MP12 and virulent ZH548 strains as well as chimeric MP12-ZH548 viruses, and in addition RVFV expressing reporter proteins.
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