Portal de Periódicos da CAPES

Molecular Cloning and Functional Characterization of a Human Scavenger Receptor with C-Type Lectin (SRCL), a Novel Member of a Scavenger Receptor Family

2001; Elsevier BV; Volume: 280; Issue: 4 Linguagem: Inglês

10.1006/bbrc.2000.4210

1090-2104

Kenji Nakamura, Hiroshi Funakoshi, Kazumasa Miyamoto, Fumio Tokunaga, Toshikazu Nakamura,

Glycosylation and Glycoproteins Research

Using a human placenta cDNA library, we cloned a novel member belonging to the scavenger receptor family. Complementary DNA of this clone encodes a type II transmembranous glycoprotein containing a collagen-like domain, which are typical structural characteristics of scavenger receptor class A. This protein also contains a C-type lectin/carbohydrate recognition domain (C-type CRD) located at the C-terminus. We designated this as Scavenger Receptor with C-type Lectin (SRCL) type I. We also isolated human SRCL type II, which lacks the C-type CRD. Northern blot analysis revealed that hSRCL type I and type II mRNAs are abundantly expressed in adult human tissues. When hSRCL type I and type II were expressed in CHO-K1 cells, they were localized in the plasma membrane forming clusters on the surface. Ligand-binding studies of CHO-K1 cells expressing hSRCL type I and type II demonstrated their specific binding capacity in Escherichia coli and Staphylococcus aureus. These results indicate that hSRCL is a novel bacteria-binding receptor containing a C-type CRD and this receptor may play an important role in host defense.