Analysis of SCEs in Vicia faba chromosomes by a simple fluorescent plus Giemsa technique
2008; BioMed Central; Volume: 107; Issue: 1 Linguagem: Inglês
10.1111/j.1601-5223.1987.tb00265.x
ISSN1601-5223
Autores Tópico(s)Carcinogens and Genotoxicity Assessment
ResumoHereditasVolume 107, Issue 1 p. 7-13 Open Access Analysis of SCEs in Vicia faba chromosomes by a simple fluorescent plus Giemsa technique F. CORTÉS, F. CORTÉS Departamento de Citologia e Histologia Vegetal y Animal, Facultad de Biologia de Sevilla, Sevilla, SpainSearch for more papers by this authorH.C. ANDERSSON, H.C. ANDERSSON Department of Genetics, University of Uppsala, Box 7003, S-7.50 07 Uppsala, SwedenSearch for more papers by this author F. CORTÉS, F. CORTÉS Departamento de Citologia e Histologia Vegetal y Animal, Facultad de Biologia de Sevilla, Sevilla, SpainSearch for more papers by this authorH.C. ANDERSSON, H.C. ANDERSSON Department of Genetics, University of Uppsala, Box 7003, S-7.50 07 Uppsala, SwedenSearch for more papers by this author First published: September 1987 https://doi.org/10.1111/j.1601-5223.1987.tb00265.xAboutPDF ToolsExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Cortés, F. and Andersson, H.C. 1987. Analysis of SCEs in Viciafaba chromosomes by a simple fluorescent plus Giemsa technique. —Hereditas 207: 7–13. Lund, Sweden. ISSN 0018–0661. Received October 3, 1986 A modified fluorescent plus Giemsa (FPG) technique for differential staining of sister chromatids and for studying sister chromatid exchanges (SCE) in root tip cells of Viciafaba is described. This new technique is quick and simple and has the advantage of a high reproducibility. The best quality of the sister chromatid differentiation (SCD) is obtained when seedlings are grown for two rounds of chromosome replication in the presence of 5-bromodeoxyuridine (BrdUrd). The technique stains the bifilarly substituted chromatid light and the unifilarly substituted one dark. However. SCD is also obtained after one round of replication in BrdUrd followed by another in the presence of thymidine. There was no difference in the baseline level of SCE in the two types of experimental protocol. The influence of various concentrations of BrdUrd on the yield of SCEs was investigated, as well as the frequency of SCEs in root tip cells grown in the absence and presence of 5-fluorodeoxyuridine. The results of these experiments are discussed. Volume107, Issue1September 1987Pages 7-13 ReferencesRelatedInformation
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