Expression of a cell‐cycle‐associated nuclear antigen (MIB 1) in cholesteatoma and auditory meatal skin
1995; Wiley; Volume: 105; Issue: 11 Linguagem: Inglês
10.1288/00005537-199511000-00017
ISSN1531-4995
AutoresHolger Sudhoff, H. Hildmann, Annette Fisseler‐Eckhoff, Jesús Bujía, Anja Holly, Christian Schulz‐Flake,
Tópico(s)RNA regulation and disease
ResumoAbstract Middle ear cholesteatoma is often invasive with consequent bone destruction. Inflammatory stimulation of the underlying connective tissue, as well as an autocrine mechanism, may be responsible for the dys‐regulation and abnormal proliferative features of the keratinocytes in cholesteatoma. Comparative investigations were performed to assess the epithelial cell kinetics of cholesteatoma and normal auditory meatal skin. Monoclonal antibody MIB 1 immuno‐staining (which recognizes a nuclear antigen expressed by dividing cells) was applied using the alkaline phosphatase antialkaline phosphatase im‐munolabeling method. Specimens of normal auditory meatal skin (n = 7) revealed an average MIB 1 score (quotient of the MIB 1‐positive cells and the total number of cells) of 7.6 ± 2.2%. Cholesteatoma samples (n = 13) showed an average MIB 1 score of 17.4 ± 8.9% and a heterogeneity of proliferating epithelial areas. Epithelial cones growing toward the underlying stroma exhibited high mitotic activity. Statistically, the results of this study confirm a highly significant increase in the proliferation rate of cholesteatoma keratinocytes, which had an MIB 1 score that was 2.3 times higher than the score for keratinocytes of normal external auditory meatal skin.
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